Investigation of corneal endothelial changes post selective laser trabeculoplasty.
Clin Exp Ophthalmol
; 46(7): 730-737, 2018 09.
Article
en En
| MEDLINE
| ID: mdl-29442432
ABSTRACT
IMPORTANCE Transient corneal endothelial changes are routinely noted on slit-lamp examination immediately following selective laser trabeculoplasty (SLT). BACKGROUND:
To determine the mechanism of transient corneal endothelial changes observed following SLT.DESIGN:
University laboratory-based observational study. SAMPLES Ten corneas from six human cadaveric donors.METHODS:
Corneas were treated with SLT, direct laser or peroxide, or used as controls. Haematoxylin and eosin staining and immunolabelling for zonula occludens-1 (ZO-1) and beta-catenin were performed. MAIN OUTCOMEMEASURES:
Histological appearance; ZO-1 and beta-catenin immunostaining.RESULTS:
There were no differences in histological features between SLT-treated and control corneas. Corneas treated with SLT or peroxide showed reduced and less regular ZO-1 immunofluorescence along cell membranes compared with ZO-1 expression in controls. These changes were generalized across the endothelium. There was no effect on the ZO-1 immunostaining after direct laser. There was no difference in beta-catenin immunostaining patterns between control, SLT and peroxide-treated corneas. CONCLUSIONS AND RELEVANCE Altered ZO-1 immunostaining may represent disassembly of tight junctions between corneal endothelial cells. The similarity of our findings between SLT-treated and peroxide-treated corneas suggests that both conditions trigger changes at the level of endothelial tight junctions, perhaps triggered by liberation of free radicals as previously proposed.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Endotelio Corneal
/
Trabeculectomía
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Glaucoma de Ángulo Abierto
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Terapia por Láser
/
Presión Intraocular
Tipo de estudio:
Diagnostic_studies
/
Observational_studies
Límite:
Adolescent
/
Adult
/
Aged
/
Aged80
/
Female
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Humans
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Male
/
Middle aged
Idioma:
En
Revista:
Clin Exp Ophthalmol
Asunto de la revista:
OFTALMOLOGIA
Año:
2018
Tipo del documento:
Article
País de afiliación:
Australia