Natural Product Diversification Mediated by Alternative Transcriptional Starting.
Angew Chem Int Ed Engl
; 57(20): 5699-5702, 2018 05 14.
Article
en En
| MEDLINE
| ID: mdl-29508935
ABSTRACT
Photorhabdus luminescens dedicates a significant proportion of its genome to the production of natural products. These products and the structural variation in their derivatives may occur by a number of well-described mechanisms, such as module skipping or precursor promiscuity. Cappable-seq was used to identify transcriptional start sites of many of the gene clusters present in P.â
luminescens TTO1. We discovered that variations associated with the non-ribosomal peptide synthetase Kol, which is responsible for kolossinâ
A production, possessed a number of internal transcripts that lead to synthesis of the smaller kolossin derivatives kolossinâ
B and C. The data here support a new mechanism of natural product biosynthetic variation whereby mRNA may code for shorter NRPS enzymes in addition to full-length proteins, resulting in the production of smaller peptide derivatives.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
3_ND
Problema de salud:
3_neglected_diseases
/
3_zoonosis
Asunto principal:
Péptido Sintasas
/
Productos Biológicos
/
Photorhabdus
Idioma:
En
Revista:
Angew Chem Int Ed Engl
Año:
2018
Tipo del documento:
Article
País de afiliación:
Alemania