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The effects of galectin-3 depletion apheresis on induced skin inflammation in a porcine model.
Navarro-Alvarez, Nalu; Goncalves, Beatriz; Andrews, Alec R; Wang, Zhaohui; Wang, Zhirui; Harrington, Edward; Shah, Jigesh; Sachs, David H; Eliaz, Isaac; Huang, Christene A.
Afiliación
  • Navarro-Alvarez N; Center for Transplantation Sciences, Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts.
  • Goncalves B; Center for Transplantation Sciences, Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts.
  • Andrews AR; Center for Transplantation Sciences, Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts.
  • Wang Z; Center for Transplantation Sciences, Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts.
  • Wang Z; Center for Transplantation Sciences, Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts.
  • Harrington E; Center for Transplantation Sciences, Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts.
  • Shah J; Center for Transplantation Sciences, Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts.
  • Sachs DH; Center for Transplantation Sciences, Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts.
  • Eliaz I; Amitabha Medical Clinic and Healing Center, Santa Rosa, California.
  • Huang CA; Center for Transplantation Sciences, Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts.
J Clin Apher ; 33(4): 486-493, 2018 Aug.
Article en En | MEDLINE | ID: mdl-29572917
ABSTRACT
Galectin-3 (Gal-3), a ß-galactoside-binding lectin that is expressed in mammalian cells, is known to modulate several biological functions such as cell-cell adhesion, macrophage activation, angiogenesis, metastasis, and fibrosis. The goal of this study was to evaluate the ability of Gal-3 depletion apheresis using an adsorption column with immobilized anti-Gal-3-antibody to reduce inflammation induced by Complete Freund's Adjuvant injection in a skin inflammation porcine model. Here, we report that plasma perfusion by apheresis through a Gal-3 binding immuno-affinity column reduces plasma Gal-3 levels to below limits of quantitative detection, and results in significant decrease in skin inflammation, including degree and duration of inflammatory lesions. Human plasma was tested ex vivo and found to be efficiently depleted using the anti-Gal-3 affinity column. This study demonstrates the potential of Gal-3 depletion apheresis as a therapeutic method for inflammation-mediated disease, supporting continued research in this area for clinical application.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Eliminación de Componentes Sanguíneos / Galectina 3 / Inflamación Límite: Animals / Humans Idioma: En Revista: J Clin Apher Año: 2018 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Eliminación de Componentes Sanguíneos / Galectina 3 / Inflamación Límite: Animals / Humans Idioma: En Revista: J Clin Apher Año: 2018 Tipo del documento: Article
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