A consensus-guided approach yields a heat-stable alkane-producing enzyme and identifies residues promoting thermostability.

Shakeel, Tabinda; Gupta, Mayank; Fatma, Zia; Kumar, Rakesh; Kumar, Raubins; Singh, Rahul; Sharma, Medha; Jade, Dhananjay; Gupta, Dinesh; Fatma, Tasneem; Yazdani, Syed Shams

Shakeel, Tabinda; Gupta, Mayank; Fatma, Zia; Kumar, Rakesh; Kumar, Raubins; Singh, Rahul; Sharma, Medha; Jade, Dhananjay; Gupta, Dinesh; Fatma, Tasneem; Yazdani, Syed Shams.

Afiliación

Shakeel T; From the Microbial Engineering Group.
Gupta M; DBT-ICGEB Centre for Advanced Bioenergy Research, International Centre for Genetic Engineering and Biotechnology, New Delhi 110067 and.
Fatma Z; From the Microbial Engineering Group.
Kumar R; DBT-ICGEB Centre for Advanced Bioenergy Research, International Centre for Genetic Engineering and Biotechnology, New Delhi 110067 and.
Kumar R; From the Microbial Engineering Group.
Singh R; DBT-ICGEB Centre for Advanced Bioenergy Research, International Centre for Genetic Engineering and Biotechnology, New Delhi 110067 and.
Sharma M; Translational Bioinformatics Group, and.
Jade D; From the Microbial Engineering Group.
Gupta D; From the Microbial Engineering Group.
Fatma T; DBT-ICGEB Centre for Advanced Bioenergy Research, International Centre for Genetic Engineering and Biotechnology, New Delhi 110067 and.
Yazdani SS; From the Microbial Engineering Group.

J Biol Chem ; 293(24): 9148-9161, 2018 06 15.

Article en En | MEDLINE | ID: mdl-29632075

Asunto(s)

Aldehídos/metabolismo; Alcanos/metabolismo; Cianobacterias/enzimología; Oxigenasas/metabolismo; Biocombustibles/microbiología; Cianobacterias/química; Cianobacterias/genética; Cianobacterias/metabolismo; Estabilidad de Enzimas; Escherichia coli/química; Escherichia coli/enzimología; Escherichia coli/genética; Escherichia coli/metabolismo; Genes Bacterianos; Manantiales de Aguas Termales/microbiología; Calor; Metagenoma; Modelos Moleculares; Mutagénesis Sitio-Dirigida/métodos; Nostoc/química; Nostoc/enzimología; Nostoc/genética; Nostoc/metabolismo; Oxigenasas/química; Oxigenasas/genética; Conformación Proteica

Palabras clave

aldehyde deformylating oxygenase; biofuel; enzyme mechanism; enzyme mutation; long-chain alkane; molecular dynamics; molecular modeling; protein engineering; structurefunction; thermostable enzyme

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Contexto en salud: 3_ND Problema de salud: 3_neglected_diseases / 3_zoonosis Asunto principal: Oxigenasas / Cianobacterias / Aldehídos / Alcanos Idioma: En Revista: J Biol Chem Año: 2018 Tipo del documento: Article

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