Screening of in vitro antimicrobial activity of plants used in traditional Indonesian medicine.
Pharm Biol
; 56(1): 287-293, 2018 Dec.
Article
en En
| MEDLINE
| ID: mdl-29656672
ABSTRACT
CONTEXT In many regions of Indonesia, there are numerous traditional herbal preparations for treatment of infectious diseases. However, their antimicrobial potential has been poorly studied by modern laboratory methods. OBJECTIVE:
This study investigates in vitro antimicrobial activity of 49 ethanol extracts from 37 plant species used in Indonesian traditional medicine for treatment against Candida albicans, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. MATERIALS ANDMETHODS:
The plants were collected from the Biopharmaca collection garden, Bogor, Indonesia. The plant material was dried, finely grounded, extracted using ethanol, concentrated, and the dried residue was dissolved in 100% DMSO. Antimicrobial activity was determined in terms of a minimum inhibitory concentration (MIC) using a broth microdilution method in 96-well microplates.RESULTS:
The extract of Orthosiphon aristatus (Blume) Miq. (Lamiaceae) leaf produced the strongest antimicrobial effect, inhibiting the growth of C. albicans (MIC 128 µg/mL), S. aureus (MIC 256 µg/mL), E. faecalis (MIC 256 µg/mL) and P. aeruginosa (MIC 256 µg/mL). The leaf extract of Woodfordia floribunda Salisb. (Lythraceae) also exhibited significant effect against C. albicans (MIC 128 µg/mL), S. aureus (MIC 256 µg/mL) and E. faecalis (MIC 256 µg/mL). Rotheca serrata (L.) Steane & Mabb. (Lamiaceae) leaf extract inhibited the growth of S. aureus (MIC 256 µg/mL) and C. albicans (MIC 256 µg/mL). DISCUSSION ANDCONCLUSIONS:
The leaf extract of O. aristatus and W. floribunda exhibited a significant anti-candidal effect. Therefore, both of these plants can serve as prospective source materials for the development of new anti-candidal agents.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Bacterias
/
Candida albicans
/
Extractos Vegetales
/
Lamiaceae
/
Woodfordia
/
Medicina Tradicional
/
Antibacterianos
/
Antifúngicos
Tipo de estudio:
Diagnostic_studies
/
Screening_studies
País/Región como asunto:
Asia
Idioma:
En
Revista:
Pharm Biol
Año:
2018
Tipo del documento:
Article
País de afiliación:
República Checa