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Single-shot super-resolution total internal reflection fluorescence microscopy.
Guo, Min; Chandris, Panagiotis; Giannini, John Paul; Trexler, Adam J; Fischer, Robert; Chen, Jiji; Vishwasrao, Harshad D; Rey-Suarez, Ivan; Wu, Yicong; Wu, Xufeng; Waterman, Clare M; Patterson, George H; Upadhyaya, Arpita; Taraska, Justin W; Shroff, Hari.
Afiliación
  • Guo M; Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA. min.guo@nih.gov.
  • Chandris P; Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA.
  • Giannini JP; Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA.
  • Trexler AJ; Biophysics Program, University of Maryland, College Park, MD, USA.
  • Fischer R; National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA.
  • Chen J; Northrop Grumman Corporation, Monterey, CA, USA.
  • Vishwasrao HD; National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA.
  • Rey-Suarez I; Advanced Imaging and Microscopy Resource, National Institutes of Health, Bethesda, MD, USA.
  • Wu Y; Advanced Imaging and Microscopy Resource, National Institutes of Health, Bethesda, MD, USA.
  • Wu X; Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA.
  • Waterman CM; Biophysics Program, University of Maryland, College Park, MD, USA.
  • Patterson GH; Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA.
  • Upadhyaya A; National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA.
  • Taraska JW; National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA.
  • Shroff H; Section on Biophotonics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA.
Nat Methods ; 15(6): 425-428, 2018 06.
Article en En | MEDLINE | ID: mdl-29735999
We combined instant structured illumination microscopy (iSIM) with total internal reflection fluorescence microscopy (TIRFM) in an approach referred to as instant TIRF-SIM, thereby improving the lateral spatial resolution of TIRFM to 115 ± 13 nm without compromising speed, and enabling imaging frame rates up to 100 Hz over hundreds of time points. We applied instant TIRF-SIM to multiple live samples and achieved rapid, high-contrast super-resolution imaging close to the coverslip surface.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Microscopía Fluorescente Límite: Humans Idioma: En Revista: Nat Methods Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Añadir a Mi BVS
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Microscopía Fluorescente Límite: Humans Idioma: En Revista: Nat Methods Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos