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Anti-band 3 and anti-spectrin antibodies are increased in Plasmodium vivax infection and are associated with anemia.
Mourão, Luiza Carvalho; Baptista, Rodrigo de Paula; de Almeida, Zélia Barbosa; Grynberg, Priscila; Pucci, Maíra Mazzoni; Castro-Gomes, Thiago; Fontes, Cor Jesus Fernandes; Rathore, Sumit; Sharma, Yagya D; da Silva-Pereira, Rosiane A; Bemquerer, Marcelo Porto; Braga, Érika Martins.
Afiliación
  • Mourão LC; Departamento de Parasitologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
  • Baptista RP; Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA, USA.
  • de Almeida ZB; Departamento de Parasitologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
  • Grynberg P; Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, Brazil.
  • Pucci MM; Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, MG, Brazil.
  • Castro-Gomes T; Departamento de Parasitologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
  • Fontes CJF; Faculdade de Ciências Médicas, Universidade Federal do Mato Grosso, Cuiabá, MT, Brazil.
  • Rathore S; Department of Biotechnology, All India Institute of Medical Sciences, New Delhi, India.
  • Sharma YD; Department of Biotechnology, All India Institute of Medical Sciences, New Delhi, India.
  • da Silva-Pereira RA; Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, MG, Brazil.
  • Bemquerer MP; Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, Brazil.
  • Braga ÉM; Departamento de Parasitologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil. embraga@icb.ufmg.br.
Sci Rep ; 8(1): 8762, 2018 06 08.
Article en En | MEDLINE | ID: mdl-29884876
Clearance of non-infected red blood cells (nRBCs) is one of the main components of anemia associated with Plasmodium vivax malaria. Recently, we have shown that anemic patients with P. vivax infection had elevated levels of anti-RBCs antibodies, which could enhance in vitro phagocytosis of nRBCs and decrease their deformability. Using immunoproteomics, here we characterized erythrocytic antigens that are differentially recognized by autoantibodies from anemic and non-anemic patients with acute vivax malaria. Protein spots exclusively recognized by anemic P. vivax-infected patients were identified by mass spectrometry revealing band 3 and spectrin as the main targets. To confirm this finding, antibody responses against these specific proteins were assessed by ELISA. In addition, an inverse association between hemoglobin and anti-band 3 or anti-spectrin antibodies levels was found. Anemic patients had higher levels of IgG against both band 3 and spectrin than the non-anemic ones. To determine if these autoantibodies were elicited because of molecular mimicry, we used in silico analysis and identified P. vivax proteins that share homology with human RBC proteins such as spectrin, suggesting that infection drives autoimmune responses. These findings suggest that band 3 and spectrin are potential targets of autoantibodies that may be relevant for P. vivax malaria-associated anemia.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Contexto en salud: 3_ND Problema de salud: 3_malaria / 3_neglected_diseases Asunto principal: Plasmodium vivax / Autoanticuerpos / Proteína 1 de Intercambio de Anión de Eritrocito / Espectrina / Malaria Vivax / Eritrocitos / Anemia Tipo de estudio: Prognostic_studies / Risk_factors_studies Límite: Adult / Humans Idioma: En Revista: Sci Rep Año: 2018 Tipo del documento: Article País de afiliación: Brasil

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Contexto en salud: 3_ND Problema de salud: 3_malaria / 3_neglected_diseases Asunto principal: Plasmodium vivax / Autoanticuerpos / Proteína 1 de Intercambio de Anión de Eritrocito / Espectrina / Malaria Vivax / Eritrocitos / Anemia Tipo de estudio: Prognostic_studies / Risk_factors_studies Límite: Adult / Humans Idioma: En Revista: Sci Rep Año: 2018 Tipo del documento: Article País de afiliación: Brasil
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