[Effect of NaHS on ATP-induced P2X receptor expression in rat microglia].
Zhongguo Ying Yong Sheng Li Xue Za Zhi
; 33(6): 519-523, 2017 Jun 08.
Article
en Zh
| MEDLINE
| ID: mdl-29931901
ABSTRACT
OBJECTIVE:
To observed the effect of sodium hydrosulphide (NaHS), a donor of H2S on the cell viability,the membrane permeability and the expression of P2X7 receptor induced by adenosine triphosphate(ATP) in rat microglia.METHODS:
Rat microglia in logarithmic growth phase was randomly divided into 4 groups. In control group, the cells were cultured without ATP treatment. In ATP group, the cells were treatment with ATP after cultured for 24 hours. In NaHS+ATP group, the cells were incubated with NaHS for 30 min before ATP, and NaHS always existed in the reaction system. In KN-62+ATP group, the cells were pretreated with KN-62 for 30 min, the others were as the same as NaHS+ATP group. The cell viability was detected by MTT. Fluorescent dyes YO-PRO-1 was used to observe the membrane permeability. The expression of P2X7 receptor was examined by immunofluorescence staining.RESULTS:
â Compared with control group, the cell viability dropped after treatment with ATP (1ã3ã5ã10 mmol/L) for 3 hours. When pre-incubation with NaHS(200 µmol/L), the cell viability was apparently higher than that of ATP alone group(P<0.01), while 400 µmol/L had no further beneficial.â¡The YO-PRO-1 fluorescence intensity was obviously elevated by ATP in rat microglia, but this effect was counteracted by NaHS pretreatment (P<0.01). ⢠The expression of P2X7 receptor protein was significantly increased after ATP(3 mmol/L) for 3 h. While the expression upregulation of P2X7 receptor protein induced by ATP was significantly counteracted by pretreating with NaHS(200 µmol/L) (P<0.01).CONCLUSIONS:
NaHS could reduce the expression of P2X7 receptor, decrease membrane permeability, and increase the cell viability in rat microglia injured by ATP. So the cytoprotection of hydrogen sulfide may be related to the expression and function of P2X7 receptor.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Sulfuros
/
Adenosina Trifosfato
/
Microglía
/
Receptores Purinérgicos P2X7
Límite:
Animals
Idioma:
Zh
Revista:
Zhongguo Ying Yong Sheng Li Xue Za Zhi
Asunto de la revista:
FISIOLOGIA
Año:
2017
Tipo del documento:
Article
País de afiliación:
China