[Development of a novel method for UPLC-QTOF-MS analysis of anti-schistosomiasis heterocyclic compounds].
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi
; 30(3): 317-321, 2018 Jun 26.
Article
en Zh
| MEDLINE
| ID: mdl-30019561
ABSTRACT
OBJECTIVE:
To develop an ultra-performance liquid chromatography/quadrupole-time of flight mass spectrometry (UPLC-QTOF-MS) method for the determination of an oxadiazole-2-oxide heterocyclic compound F-2015-14.METHODS:
Mouse plasma and liver homogenate specimens were extracted with ethyl acetate and chromatographed on a Waters CORTECS column (C18, 1.6 µm, 2.1 mm × 150 mm) by using a mobile phase of 10% acetonitrile-0.1% formic acid with by a volume fractionation by gradient elution. Then, UPLC-QTOF-MS was performed to determine F-2015-14 in mouse plasma and liver homogenate specimens.RESULTS:
The linearity of F-2015-14 in plasma ranged from 12.5 to 250 mg/mL with a correlation coefficient of 0.990 and a detection limit of 8.8 mg/mL. F-2015-14 in liver homogenates ranged from 12.5 to 250 mg/mL. The linearity was good with a correlation coefficient of 0.992 and a limit of detection of 5.6 mg/mL. If the concentration of plasma and liver homogenate specimens was 12.5 mg/mL, the accuracy and the matrix effect were 80% to 120%, and the inter-day and intra-day precision was within 20%. If the concentrations of plasma and liver homogenate specimens were 100 mg/mL and 200 mg/mL, the accuracy and the matrix effect were 85% to 115%, and the inter-day and intra-day precision was within 15%.CONCLUSIONS:
The UPLC-QTOF-MS established in this study has a high sensitivity and good reproducibility for the determination of F-2015-14, which provides bases for the development of novel anti-schistosomiasis drugs.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
3_ND
Problema de salud:
3_neglected_diseases
/
3_zoonosis
Asunto principal:
Esquistosomiasis
/
Esquistosomicidas
/
Técnicas de Química Analítica
/
Cromatografía Líquida de Alta Presión
/
Espectrometría de Masas en Tándem
/
Compuestos Heterocíclicos
Límite:
Animals
Idioma:
Zh
Revista:
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi
Año:
2018
Tipo del documento:
Article
País de afiliación:
China