Application of 2'-O-(2-N-Methylcarbamoylethyl) Nucleotides in RNase H-Dependent Antisense Oligonucleotides.
Nucleic Acid Ther
; 28(5): 307-311, 2018 10.
Article
en En
| MEDLINE
| ID: mdl-30020852
ABSTRACT
An RNase H-dependent antisense oligonucleotide (ASO), having the 2'-O-(2-N-methylcarbamoylethyl) (MCE) modification, was evaluated in vitro and in vivo. The antisense activities of an ASO having the MCE modification were comparable with those of an ASO having the 2'-O-methoxyethyl (MOE) modification in both in vitro and in vivo experiments. In contrast, the hepatotoxic potential of the ASO having the MCE modification was lower than that of the ASO having the MOE modification. Thus, these findings suggested that the MCE modification could be used as an alternative to the MOE modification.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Oligodesoxirribonucleótidos
/
ARN Mensajero
/
Oligonucleótidos Antisentido
/
Ribonucleasa H
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Nucleic Acid Ther
Año:
2018
Tipo del documento:
Article
País de afiliación:
Japón