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Ki67 is a Graded Rather than a Binary Marker of Proliferation versus Quiescence.
Miller, Iain; Min, Mingwei; Yang, Chen; Tian, Chengzhe; Gookin, Sara; Carter, Dylan; Spencer, Sabrina L.
Afiliación
  • Miller I; Department of Biochemistry, University of Colorado-Boulder, Boulder, CO 80303, USA; BioFrontiers Institute, University of Colorado-Boulder, Boulder, CO 80303, USA.
  • Min M; Department of Biochemistry, University of Colorado-Boulder, Boulder, CO 80303, USA; BioFrontiers Institute, University of Colorado-Boulder, Boulder, CO 80303, USA.
  • Yang C; Department of Biochemistry, University of Colorado-Boulder, Boulder, CO 80303, USA; BioFrontiers Institute, University of Colorado-Boulder, Boulder, CO 80303, USA; Department of Molecular, Cellular, and Developmental Biology, University of Colorado-Boulder, Boulder, CO 80303, USA.
  • Tian C; Department of Biochemistry, University of Colorado-Boulder, Boulder, CO 80303, USA; BioFrontiers Institute, University of Colorado-Boulder, Boulder, CO 80303, USA.
  • Gookin S; Department of Biochemistry, University of Colorado-Boulder, Boulder, CO 80303, USA; BioFrontiers Institute, University of Colorado-Boulder, Boulder, CO 80303, USA.
  • Carter D; Department of Biochemistry, University of Colorado-Boulder, Boulder, CO 80303, USA; BioFrontiers Institute, University of Colorado-Boulder, Boulder, CO 80303, USA.
  • Spencer SL; Department of Biochemistry, University of Colorado-Boulder, Boulder, CO 80303, USA; BioFrontiers Institute, University of Colorado-Boulder, Boulder, CO 80303, USA. Electronic address: sabrina.spencer@colorado.edu.
Cell Rep ; 24(5): 1105-1112.e5, 2018 07 31.
Article en En | MEDLINE | ID: mdl-30067968
ABSTRACT
Ki67 staining is widely used as a proliferation indicator in the clinic, despite poor understanding of this protein's function or dynamics. Here, we track Ki67 levels under endogenous control in single cells over time and find that Ki67 accumulation occurs only during S, G2, and M phases. Ki67 is degraded continuously in G1 and G0 phases, regardless of the cause of entry into G0/quiescence. Consequently, the level of Ki67 during G0 and G1 in individual cells is highly heterogeneous and depends on how long an individual cell has spent in G0. Thus, Ki67 is a graded rather than a binary marker both for cell-cycle progression and time since entry into quiescence.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ciclo Celular / Antígeno Ki-67 / Proliferación Celular Límite: Humans Idioma: En Revista: Cell Rep Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ciclo Celular / Antígeno Ki-67 / Proliferación Celular Límite: Humans Idioma: En Revista: Cell Rep Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos