Influence of Fluorescent Protein Maturation on FRET Measurements in Living Cells.

Liu, Boqun; Mavrova, Sara N; van den Berg, Jonas; Kristensen, Sebastian K; Mantovanelli, Luca; Veenhoff, Liesbeth M; Poolman, Bert; Boersma, Arnold J

Liu, Boqun; Mavrova, Sara N; van den Berg, Jonas; Kristensen, Sebastian K; Mantovanelli, Luca; Veenhoff, Liesbeth M; Poolman, Bert; Boersma, Arnold J.

Afiliación

Liu B; Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute , University of Groningen , Nijenborgh 4 , 9747 AG Groningen , The Netherlands.
Mavrova SN; Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute , University of Groningen , Nijenborgh 4 , 9747 AG Groningen , The Netherlands.
van den Berg J; Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute , University of Groningen , Nijenborgh 4 , 9747 AG Groningen , The Netherlands.
Kristensen SK; Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute , University of Groningen , Nijenborgh 4 , 9747 AG Groningen , The Netherlands.
Mantovanelli L; Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute , University of Groningen , Nijenborgh 4 , 9747 AG Groningen , The Netherlands.
Veenhoff LM; European Research Institute for the Biology of Ageing , University of Groningen , University Medical Center Groningen, 9713 AV Groningen , The Netherlands.
Poolman B; Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute , University of Groningen , Nijenborgh 4 , 9747 AG Groningen , The Netherlands.
Boersma AJ; Zernike Institute for Advanced Materials , University of Groningen , Nijenborgh 4 , 9747 AG Groningen , The Netherlands.

ACS Sens ; 3(9): 1735-1742, 2018 09 28.

Article en En | MEDLINE | ID: mdl-30168711

Asunto(s)

Transferencia Resonante de Energía de Fluorescencia/métodos; Proteínas Fluorescentes Verdes/metabolismo; Secuencia de Bases; Fenómenos Fisiológicos Celulares; Cloranfenicol/farmacología; Escherichia coli/citología; Escherichia coli/genética; Regulación de la Expresión Génica; Proteínas Fluorescentes Verdes/genética; Proteínas de Unión a Maltosa/metabolismo; Microscopía Confocal/métodos; Microscopía Fluorescente/métodos; Modelos Biológicos; Mutación; Regiones Promotoras Genéticas; Inhibidores de la Síntesis de la Proteína/farmacología; Saccharomyces cerevisiae/citología; Saccharomyces cerevisiae/genética

Palabras clave

FRET sensors; Förster resonance energy transfer; biosensors; fluorescent protein maturation; macromolecular crowding

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Contexto en salud: 3_ND Problema de salud: 3_neglected_diseases / 3_zoonosis Asunto principal: Transferencia Resonante de Energía de Fluorescencia / Proteínas Fluorescentes Verdes Idioma: En Revista: ACS Sens Año: 2018 Tipo del documento: Article País de afiliación: Países Bajos

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