Cytotoxicity analysis of staphylococcal bi-component ß-pore forming toxins using the CHO cells expressing human lymphocyte receptor CCR5.

Peng, Zhao; Shibata, Nao; Tada, Hideaki; Kaneko, Jun

Peng, Zhao; Shibata, Nao; Tada, Hideaki; Kaneko, Jun.

Afiliación

Peng Z; a Department of Microbial Biotechnology , Graduate School of Agricultural Science, Tohoku University , Sendai , Japan.
Shibata N; b Exploratory Research Laboratories , Tsukuba Research Institute, ONO pharmaceutical Co., LTD , Tsukuba , Japan.
Tada H; b Exploratory Research Laboratories , Tsukuba Research Institute, ONO pharmaceutical Co., LTD , Tsukuba , Japan.
Kaneko J; a Department of Microbial Biotechnology , Graduate School of Agricultural Science, Tohoku University , Sendai , Japan.

Biosci Biotechnol Biochem ; 82(12): 2094-2097, 2018 Dec.

Article en En | MEDLINE | ID: mdl-30185128

ABSTRACT

ABSTRACT

CCR5-mediated cytotoxicity of staphylococcal bi-component toxins was investigated using human CCR5-expressing CHO cells. Cytotoxicity of rim domain loop-exchange mutants between LukE and Hlg2 indicated that loop-4 of LukE is essential for cytotoxicity in combination with LukD. Interestingly, Hlg2 showed LukF-dependent CCR5-mediated cytotoxicity, suggesting that the F-components of toxins also play a role in the cell-specific cytotoxicity.

Asunto(s)

Proteínas Bacterianas/toxicidad; Toxinas Bacterianas/toxicidad; Exotoxinas/toxicidad; Proteínas Hemolisinas/toxicidad; Linfocitos/metabolismo; Receptores CCR5/metabolismo; Staphylococcus aureus/metabolismo; Animales; Proteínas Bacterianas/metabolismo; Toxinas Bacterianas/metabolismo; Células CHO; Supervivencia Celular/efectos de los fármacos; Cricetulus; Exotoxinas/metabolismo; Proteínas Hemolisinas/metabolismo; Humanos; Mutación; Receptores CCR5/genética

Palabras clave

CCR5; Staphylococcal ß-PFTs; cytotoxicity

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Staphylococcus aureus / Proteínas Bacterianas / Toxinas Bacterianas / Linfocitos / Receptores CCR5 / Exotoxinas / Proteínas Hemolisinas Límite: Animals / Humans Idioma: En Revista: Biosci Biotechnol Biochem Asunto de la revista: BIOQUIMICA / BIOTECNOLOGIA Año: 2018 Tipo del documento: Article País de afiliación: Japón

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