Defining Lineage-Specific Membrane Fluidity Signatures that Regulate Adhesion Kinetics.
Stem Cell Reports
; 11(4): 852-860, 2018 10 09.
Article
en En
| MEDLINE
| ID: mdl-30197117
ABSTRACT
Cellular membrane fluidity is a critical modulator of cell adhesion and migration, prompting us to define the systematic landscape of lineage-specific cellular fluidity throughout differentiation. Here, we have unveiled membrane fluidity landscapes in various lineages ranging from human pluripotency to differentiated progeny (1) membrane rigidification precedes the exit from pluripotency, (2) membrane composition modulates activin signaling transmission, and (3) signatures are relatively germ layer specific presumably due to unique lipid compositions. By modulating variable lineage-specific fluidity, we developed a label-free "adhesion sorting (AdSort)" method with simple cultural manipulation, effectively eliminating pluripotent stem cells and purifying target population as a result of the over 1,150 of screened conditions combining compounds and matrices. These results underscore the important role of tunable membrane fluidity in influencing stem cell maintenance and differentiation that can be translated into lineage-specific cell purification strategy.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Linaje de la Célula
/
Fluidez de la Membrana
Límite:
Humans
Idioma:
En
Revista:
Stem Cell Reports
Año:
2018
Tipo del documento:
Article
País de afiliación:
Japón