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RBM17 controls apoptosis and proliferation to promote Glioma progression.
Lu, Jianglong; Li, Qun; Cai, Lin; Zhu, Zhangzhang; Guan, Jiaqing; Wang, Chunyong; Xia, Jia; Xia, Lei; Wen, Min; Zheng, Weiming; Su, Zhipeng; Wang, Chengde.
Afiliación
  • Lu J; Department of Neurosurgery, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China.
  • Li Q; Department of Neurosurgery, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China.
  • Cai L; Department of Neurosurgery, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China.
  • Zhu Z; Department of Neurosurgery, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China.
  • Guan J; Department of Neurosurgery, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China.
  • Wang C; Department of Neurosurgery, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China.
  • Xia J; Department of Neurosurgery, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China.
  • Xia L; Department of Neurosurgery, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China.
  • Wen M; Department of Neurosurgery, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China.
  • Zheng W; Department of Neurosurgery, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China.
  • Su Z; Department of Neurosurgery, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China. Electronic address: drsuzhipeng@163.com.
  • Wang C; Department of Neurosurgery, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China. Electronic address: yihe723@126.com.
Biochem Biophys Res Commun ; 505(1): 20-28, 2018 10 20.
Article en En | MEDLINE | ID: mdl-30227940
The splicing factor SPF45 (RBM17) is a well-known component of the spliceosome that is involved in alternative splicing. RBM17 is frequently overexpressed in many tumors and plays a crucial role in cancer progression and drug resistance. However, the role of RBM17 in the development of glioma has not been thoroughly elucidated to date. In the present study, we found that RBM17 was overexpressed in glioma and that a high level of expression of RBM17 was closely associated with a poor prognosis in glioma patients. We investigated the effect of RBM17 on apoptosis, cell growth and cell cycle indexes and the activation of apoptosis signaling by shRNA in human U87 and U251 glioma cells. The downregulated expression of RBM17 mRNA was accompanied by the induction of cell cycle arrest, and apoptosis, reduced cell proliferation in the two cell lines, and reduced cell survival, as measured by the increased activation of caspase-3, caspase-9, and PARP (poly ADP-ribose polymerase). Furthermore, in subcutaneous U87 cell xenograft tumors in nude mice, intradermal administration of an shRNA targeting RBM17 significantly downregulated RBM17 expression in vivo and was accompanied by the suppressed growth of glioma. To the best of our knowledge, our results are the first to confirm that RBM17 functions in promoting cell proliferation, affecting the cell cycle, and inducing apoptosis in human glioma cells both in vitro and in vivo. These results indicate that RBM17 may be a therapeutic target in the clinical management of glioma.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Contexto en salud: 1_ASSA2030 / 6_ODS3_enfermedades_notrasmisibles Problema de salud: 1_doencas_nao_transmissiveis / 6_brain_nervous_system_cancer Asunto principal: Neoplasias Encefálicas / Regulación Neoplásica de la Expresión Génica / Apoptosis / Proliferación Celular / Factores de Empalme de ARN / Glioma Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Biochem Biophys Res Commun Año: 2018 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Contexto en salud: 1_ASSA2030 / 6_ODS3_enfermedades_notrasmisibles Problema de salud: 1_doencas_nao_transmissiveis / 6_brain_nervous_system_cancer Asunto principal: Neoplasias Encefálicas / Regulación Neoplásica de la Expresión Génica / Apoptosis / Proliferación Celular / Factores de Empalme de ARN / Glioma Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Biochem Biophys Res Commun Año: 2018 Tipo del documento: Article País de afiliación: China
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