Discovery of multi-megabase polymorphic inversions by chromosome conformation capture sequencing in large-genome plant species.
Plant J
; 96(6): 1309-1316, 2018 12.
Article
en En
| MEDLINE
| ID: mdl-30256471
ABSTRACT
Chromosomal inversions occur in natural populations of many species, and may underlie reproductive isolation and local adaptation. Traditional methods of inversion discovery are labor-intensive and lack sensitivity. Here, we report the use of three-dimensional contact probabilities between genomic loci as assayed by chromosome-conformation capture sequencing (Hi-C) to detect multi-megabase polymorphic inversions in four barley genotypes. Inversions are validated by fluorescence in situ hybridization and Bionano optical mapping. We propose Hi-C as a generally applicable method for inversion discovery in natural populations.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Hordeum
/
Genoma de Planta
/
Cromosomas de las Plantas
/
Inversión Cromosómica
Idioma:
En
Revista:
Plant J
Asunto de la revista:
BIOLOGIA MOLECULAR
/
BOTANICA
Año:
2018
Tipo del documento:
Article
País de afiliación:
Alemania