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Epigenetic influences on aging: a longitudinal genome-wide methylation study in old Swedish twins.
Wang, Yunzhang; Karlsson, Robert; Lampa, Erik; Zhang, Qian; Hedman, Åsa K; Almgren, Malin; Almqvist, Catarina; McRae, Allan F; Marioni, Riccardo E; Ingelsson, Erik; Visscher, Peter M; Deary, Ian J; Lind, Lars; Morris, Tiffany; Beck, Stephan; Pedersen, Nancy L; Hägg, Sara.
Afiliación
  • Wang Y; a Department of Medical Epidemiology and Biostatistics , Karolinska Institutet , Stockholm , Sweden.
  • Karlsson R; a Department of Medical Epidemiology and Biostatistics , Karolinska Institutet , Stockholm , Sweden.
  • Lampa E; b Department of Medical Sciences , Cardiovascular Epidemiology, Uppsala University , Uppsala , Sweden.
  • Zhang Q; c Institute for Molecular Bioscience , The University of Queensland , Brisbane , Australia.
  • Hedman ÅK; d Department of Medical Sciences , Molecular Epidemiology and Science for Life Laboratory, Uppsala University , Uppsala , Sweden.
  • Almgren M; e Cardiovascular Medicine unit, Department of Medicine Solna , Karolinska Institute , Stockholm , Sweden.
  • Almqvist C; f Department of Clinical Neuroscience , Centrum for Molecular Medicine, Karolinska Institutet , Stockholm , Sweden.
  • McRae AF; a Department of Medical Epidemiology and Biostatistics , Karolinska Institutet , Stockholm , Sweden.
  • Marioni RE; g Astrid Lindgren Children's Hospital, Karolinska University Hospital , Stockholm , Sweden.
  • Ingelsson E; c Institute for Molecular Bioscience , The University of Queensland , Brisbane , Australia.
  • Visscher PM; h Centre for Genomic and Experimental Medicine, Institute of Genetics and Molecular Medicine , University of Edinburgh , Edinburgh , United Kingdom.
  • Deary IJ; i Centre for Cognitive Ageing and Cognitive Epidemiology, Department of Psychology , University of Edinburgh , Edinburgh , United Kingdom.
  • Lind L; d Department of Medical Sciences , Molecular Epidemiology and Science for Life Laboratory, Uppsala University , Uppsala , Sweden.
  • Morris T; j Department of Medicine, Division of Cardiovascular Medicine , Stanford University School of Medicine , Stanford , CA , USA.
  • Beck S; k Stanford Cardiovascular Institute , Stanford University , Stanford , CA , USA.
  • Pedersen NL; c Institute for Molecular Bioscience , The University of Queensland , Brisbane , Australia.
  • Hägg S; l The Queensland Brain Institute , The University of Queensland , St Lucia , Brisbane , Australia.
Epigenetics ; 13(9): 975-987, 2018.
Article en En | MEDLINE | ID: mdl-30264654
Age-related changes in DNA methylation were observed in cross-sectional studies, but longitudinal evidence is still limited. Here, we aimed to characterize longitudinal age-related methylation patterns using 1011 blood samples collected from 385 Swedish twins (age at entry: mean 69 and standard deviation 9.7, 73 monozygotic and 96 dizygotic pairs) up to five times (mean 2.6) over 20 years (mean 8.7). We identified 1316 age-associated methylation sites (P<1.3×10-7) using a longitudinal epigenome-wide association study design. We measured how estimated cellular compositions changed with age and how much they confounded the age effect. We validated the results in two independent longitudinal cohorts, where 118 CpGs were replicated in Prospective Investigation of the Vasculature in Uppsala Seniors (PIVUS, 390 samples) (P<3.9×10-5), 594 in Lothian Birth Cohort (LBC, 3018 samples) (P<5.1×10-5) and 63 in both. Functional annotation of age-associated CpGs showed enrichment in CCCTC-binding factor (CTCF) and other transcription factor binding sites. We further investigated genetic influences on methylation and found no interaction between age and genetic effects in the 1316 age-associated CpGs. Moreover, in the same CpGs, methylation differences within twin pairs increased with 6.4% over 10 years, where monozygotic twins had smaller intra-pair differences than dizygotic twins. In conclusion, we show that age-related methylation changes persist in a longitudinal perspective, and are fairly stable across cohorts. The changes are under genetic influence, although this effect is independent of age. Moreover, methylation variability increase over time, especially in age-associated CpGs, indicating the increase of environmental contributions on DNA methylation with age.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Envejecimiento / Metilación de ADN / Epigénesis Genética Tipo de estudio: Observational_studies / Prognostic_studies / Risk_factors_studies Límite: Aged / Female / Humans / Male Idioma: En Revista: Epigenetics Asunto de la revista: GENETICA Año: 2018 Tipo del documento: Article País de afiliación: Suecia

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Envejecimiento / Metilación de ADN / Epigénesis Genética Tipo de estudio: Observational_studies / Prognostic_studies / Risk_factors_studies Límite: Aged / Female / Humans / Male Idioma: En Revista: Epigenetics Asunto de la revista: GENETICA Año: 2018 Tipo del documento: Article País de afiliación: Suecia
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