Enhancing Checkpoint Inhibitor Therapy with Ultrasound Stimulated Microbubbles.
Ultrasound Med Biol
; 45(2): 500-512, 2019 02.
Article
en En
| MEDLINE
| ID: mdl-30447880
ABSTRACT
Checkpoint inhibitor (CI) immunotherapy is playing an increasingly prominent role in the treatment of cancer but is effective and durable in only a subset of patients. There are concerted efforts to improve CI therapy through the use of multiple CIs or use of CIs in combination with other anti-cancer agents. Here we investigate the use of "anti-vascular" ultrasound-stimulated microbubble (USMB) treatments in combination with anti-PD-1 CI therapy. The colorectal cancer cell line CT26 was used to conduct longitudinal growth studies along with acute experiments to assess ultrasound-induced anti-tumor immune responses using flow cytometry and enzyme-linked immunospot (ELISPOT) analysis. Longitudinal experiments indicated that USMBâ¯+â¯anti-PD-1 treatments significantly enhanced tumor growth inhibition and animal survival relative to monotherapies. Flow cytometry and ELISPOT data did not clearly support a T cell-dependent mechanism for the enhancement. Therefore, the results indicate the ability of anti-vascular USMBs to increase the anti-tumor effects of CI therapy; the specific mechanisms of enhancement remain to be established.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ultrasonido
/
Neoplasias Colorrectales
/
Microburbujas
/
Inmunoterapia
Límite:
Animals
Idioma:
En
Revista:
Ultrasound Med Biol
Año:
2019
Tipo del documento:
Article
País de afiliación:
Canadá