Homogeneity of Different Functional mRNA Indicators for Wound Age Estimation.
Fa Yi Xue Za Zhi
; 34(5): 487-491, 2018 Oct.
Article
en En, Zh
| MEDLINE
| ID: mdl-30468050
ABSTRACT
OBJECTIVES:
To explore the homogeneity level of four different functional mRNA ï¼PUM2, TAB2, Cx45 and CHRNA1ï¼ expressions in rats with skeletal muscle contusion.METHODS:
The relative expressions of PUM2, TAB2, Cx45 and CHRNA1 mRNAs were detected by quantitative real-time reverse transcription-polymerase chain reaction ï¼RT-qPCRï¼. The coefficient of variation ï¼CVï¼ of the relative expressions for different individuals in each injury group was calculated. The extreme value of CV, cumulative variability, and CVCV were compared.RESULTS:
A high CV of PUM2 and TAB2 mRNAs appeared on several different time points. However, the CV of Cx45 and CHRNA1 mRNAs was relatively low. The cumulative variability from high to low was PUM2, CHRNA1, TAB2 and Cx45 mRNAs. The relative expression of PUM2 mRNA was significantly higher than that of TAB2, Cx45 and CHRNA1 mRNAs ï¼ P<0.05ï¼. There was no statistical significance ï¼P>0.05ï¼ in the CVCV of the relative expression of TAB2, CHRNA1 and Cx45 mRNAs.CONCLUSIONS:
As the mRNAs involving in biological process regulation, PUM2 and CHRNA1 mRNAs show a lowest individual homogeneity of the relative expression followed by TAB2 mRNA. As the mRNAs participating in the composition of cellular structure, Cx45 and CHRNA1 mRNAs show a high individual homogeneity of the relative expressions. The functional classification should be considered for the screening of the mRNA indicators used for wound age estimation.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Cicatrización de Heridas
/
ARN Mensajero
/
Proteínas de Unión al ARN
/
Contusiones
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
/
Zh
Revista:
Fa Yi Xue Za Zhi
Asunto de la revista:
JURISPRUDENCIA
Año:
2018
Tipo del documento:
Article
País de afiliación:
China