An On-Off-On Fluorescence Probe Based on Coumarin for Cu2+, Cysteine, and Histidine Detections.
Appl Spectrosc
; 73(7): 794-800, 2019 Jul.
Article
en En
| MEDLINE
| ID: mdl-30523694
ABSTRACT
A simple coumarin-based Schiff base (probe L) was successfully developed. It showed strong green fluorescence emission at 527 nm with a 70.3% of fluorescence quantum efficiency (ΦF). However, after the addition of common metal ions, probe L can only combine with Cu2+ ions and displayed significant fluorescence quenching of > 96.2% (ΦF = 2.7%) due to the paramagnetic quenching action from Cu2+. Conversely, by the coordination action of cysteine (Cys) and histidine (His), the quenching fluorescence of the complex (L-Cu2+) between probe L and Cu2+ ions was recovered mostly because the Cys and His can usurp Cu2+ of L-Cu2+ and led to the liberation of probe L. Based on the fluorescence changes of probe L with the actions of Cu2+, Cys, and His, an on-off-on reversible fluorescence probe for sensitive and specific monitoring Cu2+, Cys, and His has been prepared. More importantly, the probe L and L-Cu2+ ensemble can be used, respectively, to test Cu2+ and Cys/His in live cells and human urine samples with great reliability.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Bases de Schiff
/
Espectrometría de Fluorescencia
/
Técnicas Biosensibles
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Cobre
/
Cumarinas
/
Cisteína
/
Histidina
Límite:
Humans
Idioma:
En
Revista:
Appl Spectrosc
Año:
2019
Tipo del documento:
Article
País de afiliación:
China