Systematic deletions in the cellobiohydrolase (CBH) Cel7A from the fungus <i>Trichoderma reesei</i> reveal flexible loops critical for CBH activity.

Schiano-di-Cola, Corinna; Røjel, Nanna; Jensen, Kenneth; Kari, Jeppe; Sørensen, Trine Holst; Borch, Kim; Westh, Peter

Systematic deletions in the cellobiohydrolase (CBH) Cel7A from the fungus Trichoderma reesei reveal flexible loops critical for CBH activity.

Schiano-di-Cola, Corinna; Røjel, Nanna; Jensen, Kenneth; Kari, Jeppe; Sørensen, Trine Holst; Borch, Kim; Westh, Peter.

Afiliación

Schiano-di-Cola C; From the Department of Science and Environment, Roskilde University, Universitetsvej 1, Building 28, DK-4000 Roskilde, Denmark.
Røjel N; From the Department of Science and Environment, Roskilde University, Universitetsvej 1, Building 28, DK-4000 Roskilde, Denmark.
Jensen K; Novozymes A/S, Krogshøjvej 36, DK-2880 Bagsværd, Denmark, and.
Kari J; From the Department of Science and Environment, Roskilde University, Universitetsvej 1, Building 28, DK-4000 Roskilde, Denmark.
Sørensen TH; From the Department of Science and Environment, Roskilde University, Universitetsvej 1, Building 28, DK-4000 Roskilde, Denmark.
Borch K; Novozymes A/S, Krogshøjvej 36, DK-2880 Bagsværd, Denmark, and.
Westh P; the Department of Biotechnology and Biomedicine, Technical University of Denmark, Building 224, DK-2800 Kgs. Lyngby, Denmark petwe@dtu.dk.

J Biol Chem ; 294(6): 1807-1815, 2019 02 08.

Article en En | MEDLINE | ID: mdl-30538133

Asunto(s)

Secuencia de Aminoácidos; Celulosa 1,4-beta-Celobiosidasa; Proteínas Fúngicas; Eliminación de Secuencia; Trichoderma; Celulosa 1,4-beta-Celobiosidasa/química; Celulosa 1,4-beta-Celobiosidasa/genética; Cristalografía por Rayos X; Proteínas Fúngicas/química; Proteínas Fúngicas/genética; Estructura Secundaria de Proteína; Trichoderma/enzimología; Trichoderma/genética

Palabras clave

Cel7A; Cel7B; Trichoderma reesei; cellobiohydrolase; cellulase; cellulose; endoglucanase; enzyme kinetics; loop engineering; protein engineering

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trichoderma / Proteínas Fúngicas / Secuencia de Aminoácidos / Eliminación de Secuencia / Celulosa 1,4-beta-Celobiosidasa Idioma: En Revista: J Biol Chem Año: 2019 Tipo del documento: Article País de afiliación: Dinamarca

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