Immunogenic protein MPB57 from Mycobacterium bovis BCG: molecular cloning, nucleotide sequence and expression.
FEBS Lett
; 240(1-2): 115-7, 1988 Nov 21.
Article
en En
| MEDLINE
| ID: mdl-3056744
ABSTRACT
Using a single-probe method, we have cloned the gene for an immunogenic MPB57 protein of Mycobacterium bovis BCG. The nucleotide sequence includes an ORF of 300 base pairs encoding a protein of 99 amino acids with an Mr of 10,818. This cloned gene was expressed in an Escherichia coli expression vector to give a mature protein which reacted with a polyclonal antibody raised against MPB57.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Bacterianas
/
Mycobacterium bovis
/
Antígenos Bacterianos
Idioma:
En
Revista:
FEBS Lett
Año:
1988
Tipo del documento:
Article
País de afiliación:
Japón