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A Comprehensive Proteome Analysis of Peripheral Blood Mononuclear Cells (PBMCs) to Identify Candidate Biomarkers of Pancreatic Cancer.
Li, Hengchao; Mao, Yishen; Xiong, Yueting; Zhao, Huan Huan; Shen, Fenglin; Gao, Xing; Yang, Pengyuan; Liu, Xiaohui; Fu, Deliang.
Afiliación
  • Li H; Department of Pancreatic Surgery, Huashan Hospital, Fudan University, Shanghai, P.R. China.
  • Mao Y; Department of Pancreatic Surgery, Huashan Hospital, Fudan University, Shanghai, P.R. China.
  • Xiong Y; Institute of Biomedical Sciences, Fudan University, Shanghai, P.R. China.
  • Zhao HH; Institute of Biomedical Sciences, Fudan University, Shanghai, P.R. China.
  • Shen F; Institute of Biomedical Sciences, Fudan University, Shanghai, P.R. China.
  • Gao X; Institute of Biomedical Sciences, Fudan University, Shanghai, P.R. China.
  • Yang P; The Fifth People's Hospital of Shanghai, Fudan University, Shanghai, P.R. China.
  • Liu X; Institute of Biomedical Sciences, Fudan University, Shanghai, P.R. China pyyang@fudan.edu.cn liuxiaohui@fudan.edu.cn surgeonfu@163.com.
  • Fu D; The Fifth People's Hospital of Shanghai, Fudan University, Shanghai, P.R. China.
Cancer Genomics Proteomics ; 16(1): 81-89, 2019.
Article en En | MEDLINE | ID: mdl-30587502
ABSTRACT
BACKGROUND/

AIM:

Pancreatic cancer (PC) is currently the fourth leading cause of cancer-related mortality worldwide. Peripheral blood mononuclear cells (PBMCs) is a subpopulation of accessible and functional immune cells. Comparative analysis of the proteome of PBMCs can help us elucidate the mechanism of disease and find potential biomarkers for diagnosis. MATERIALS AND

METHODS:

PBMCs were collected from healthy individuals, patients with benign diseases, and pancreatic cancer. iTRAQ-2DLC-MS/MS and SWATH methodologies were applied to make a comparative proteomics analysis of PBMCs.

RESULTS:

A total of 3,357 proteins with a false discovery rate (FDR) <1% were identified, of which 114 proteins were found dysregulated in the PC group. An extensive SWATH library was constructed which showed a potential application for large scale clinical sample analysis.

CONCLUSION:

A PBMCs proteome with extensive protein representation was achieved, which will potentially allow the identification of novel biomarkers for PC.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Pancreáticas / Leucocitos Mononucleares / Biomarcadores de Tumor / Proteoma / Proteómica Límite: Humans Idioma: En Revista: Cancer Genomics Proteomics Asunto de la revista: BIOQUIMICA / GENETICA MEDICA / NEOPLASIAS Año: 2019 Tipo del documento: Article
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Pancreáticas / Leucocitos Mononucleares / Biomarcadores de Tumor / Proteoma / Proteómica Límite: Humans Idioma: En Revista: Cancer Genomics Proteomics Asunto de la revista: BIOQUIMICA / GENETICA MEDICA / NEOPLASIAS Año: 2019 Tipo del documento: Article