Peptide-functionalized supported lipid bilayers to construct cell membrane mimicking interfaces.
Colloids Surf B Biointerfaces
; 176: 18-26, 2019 Apr 01.
Article
en En
| MEDLINE
| ID: mdl-30590345
ABSTRACT
Supported lipid bilayers (SLB) functionalized with bioactive molecules can be effectively used to study the interaction of cells with different molecules for fundamental research or to develop biosynthetic systems for various biomedical applications. In this study, RGD and Osteocalcin mimetic (OSN) peptides were used as model molecules for functionalization of otherwise passive SLBs to evaluate cell-surface interactions via real-time monitoring in quartz crystal microbalance with dissipation. Similar platforms were also used in cell culture environment. It was seen that low density of mobile RGD peptides on SLB platforms preserved their biological activity and promoted cell adhesion more efficiently than high number of immobile, physisorbed peptides. Even though nonspecific protein and cell attachment was promoted, cells did not spread well on OSN-coated control surfaces. The stability of SLBs produced with different lipids were evaluated in various medium conditions. Enrichment with different lipids increased the stability of SLB to pure PC bilayer.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Péptidos
/
Membrana Dobles de Lípidos
Límite:
Humans
Idioma:
En
Revista:
Colloids Surf B Biointerfaces
Asunto de la revista:
QUIMICA
Año:
2019
Tipo del documento:
Article
País de afiliación:
Turquía