Selective profiling of ribosomes associated with yeast Upf proteins.
Methods
; 155: 58-67, 2019 02 15.
Article
en En
| MEDLINE
| ID: mdl-30593864
ABSTRACT
Ribosomes associated with nonsense-mediated decay factors Upf1, Upf2, or Upf3 were purified by immunoprecipitation, and enrichment and stoichiometry of Upf factors and ribosomal proteins were analyzed by western blot and mass spectrometry. Using a small RNA library preparation protocol that eliminates in-gel RNA and cDNA size selection and incorporates four random nucleotides on each side of the ribosome-protected RNA fragment allowed recovery, detection, and analysis of all size classes of protected fragments from a sample simultaneously.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ribosomas
/
Saccharomyces cerevisiae
/
ARN de Hongos
/
ARN Mensajero
/
Regulación Fúngica de la Expresión Génica
/
ARN Helicasas
/
Proteínas de Saccharomyces cerevisiae
Tipo de estudio:
Risk_factors_studies
Idioma:
En
Revista:
Methods
Asunto de la revista:
BIOQUIMICA
Año:
2019
Tipo del documento:
Article
País de afiliación:
Estados Unidos