Establishment of an ectodermal dysplasia related gene EDA Knockout human embryonic stem cell line (WAe001-A-22) by CRISPR-Cas9 technology.
Stem Cell Res
; 34: 101379, 2019 01.
Article
en En
| MEDLINE
| ID: mdl-30605838
ABSTRACT
EDA is a gene located at Xq13.1. It encodes different isoforms of tumor necrosis factor (TNF) superfamily member ectodysplasin A. Ectodysplasin A is a transmembrane protein which can be cleaved to form a secreted form and interact with EDA receptor to mediate the development of ectoderm. Mutations of the EDA gene are related to ectodermal dysplasia and tooth agenesis. Here, we report the establishment of the EDA gene knockout human embryonic stem (hES) cell line by CRISPR-Cas9 technology. This cell line provides good materials for further studies of the roles ectodysplasin A plays in ectoderm differentiation and tooth development.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Displasia Ectodérmica
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Técnicas de Cultivo de Célula
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Técnicas de Inactivación de Genes
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Sistemas CRISPR-Cas
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Células Madre Embrionarias Humanas
Límite:
Humans
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Male
Idioma:
En
Revista:
Stem Cell Res
Año:
2019
Tipo del documento:
Article
País de afiliación:
China