[Preparation and identification of monoclonal antibodies against African horse sickness virus VP7].

DU, Fangyuan; Feng, Chunyan; Wang, Caixia; Qiu, Songyin; Zhang, Yongning; Lin, Xiangmei; Wu, Shaoqiang

DU, Fangyuan; Feng, Chunyan; Wang, Caixia; Qiu, Songyin; Zhang, Yongning; Lin, Xiangmei; Wu, Shaoqiang.

Afiliación

DU F; Institute of Animal Quarantine, Chinese Academy of Inspection and Quarantine, Beijing 100176, China.
Feng C; Institute of Animal Quarantine, Chinese Academy of Inspection and Quarantine, Beijing 100176, China.
Wang C; Institute of Animal Quarantine, Chinese Academy of Inspection and Quarantine, Beijing 100176, China.
Qiu S; Institute of Animal Quarantine, Chinese Academy of Inspection and Quarantine, Beijing 100176, China.
Zhang Y; Institute of Animal Quarantine, Chinese Academy of Inspection and Quarantine, Beijing 100176, China.
Lin X; Institute of Animal Quarantine, Chinese Academy of Inspection and Quarantine, Beijing 100176, China.
Wu S; Institute of Animal Quarantine, Chinese Academy of Inspection and Quarantine, Beijing 100176, China. *Corresponding author, E-mail: wusq@caiq.gov.cn.

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 34(12): 1125-1129, 2018 Dec.

Article en Zh | MEDLINE | ID: mdl-30626480

ABSTRACT

ABSTRACT

Objective To prepare the monoclonal antibody (mAb) against the African horsefever virus (AHSV) VP7 protein and to identify it. Methods mAbs were prepared by using baculovirus expressed VP7 protein in BALB/c mice, and the effect of mAb was detected by ELISA, indirect immunofluorescence assay (IFA), and AHSV positive serum blockade. Results Four mAb strains were selected, including 20A8, 28B3, 30G8 and 47E6, among which 47E6 had the best blocking effect. Conclusion mAbs were successfully prepared against VP7 protein.

Asunto(s)

Virus de la Enfermedad Equina Africana/inmunología; Anticuerpos Monoclonales/biosíntesis; Anticuerpos Antivirales/biosíntesis; Antígenos Virales/inmunología; Proteínas del Núcleo Viral/inmunología; Animales; Caballos; Ratones; Ratones Endogámicos BALB C

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas del Núcleo Viral / Virus de la Enfermedad Equina Africana / Anticuerpos Monoclonales / Anticuerpos Antivirales / Antígenos Virales Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: Zh Revista: Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2018 Tipo del documento: Article País de afiliación: China

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