Detection of Brucella abortus by a platform functionalized with protein A and specific antibodies IgG.
Microsc Res Tech
; 82(5): 586-595, 2019 May.
Article
en En
| MEDLINE
| ID: mdl-30637865
ABSTRACT
Oriented immobilization of antibodies on a sensor surface is critical for enhancing both the antigen-binding capacity and the sensitivity of immunosensors. In this study, we describe a strategy to adsorb immunoglobulin G (IgG) anti-Brucella antibodies onto a silicon surface, oriented by protein A obtained from Staphylococcus aureus (SpA). X-ray photoelectron spectroscopy and atomic force microscopy were used to characterize topographically, morphologically, and chemical changes of the sensor functionalization. The activity of the biosensor was assessed by confocal microscopy, scanning electronic microscopy, and bacteria capture assays (BCA). According to the BCA, the efficiency of Brucella abortus detection with the SpA-IgG anti Brucella biosensor was three-fold higher than that of the random orientated IgG anti Brucella biosensor. The limit of detection was 1 × 106 CFU/ml. These data show that the orientation of antibodies immobilization is crucial to developing immunosensors for bacterial antigen detection as Brucella spp and improve its sensibility level. Functionalization with protein A increases Brucella detection by an antibody-coated surface. Functionalized silicon surface for Brucella detection was characterized by atomic force microscopy, X-ray photoelectron spectroscopy and confocal microscopy.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
3_ND
Problema de salud:
3_zoonosis
Asunto principal:
Brucella abortus
/
Inmunoensayo
/
Técnicas Biosensibles
/
Anticuerpos Inmovilizados
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Microsc Res Tech
Asunto de la revista:
DIAGNOSTICO POR IMAGEM
Año:
2019
Tipo del documento:
Article
País de afiliación:
México