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Guidelines for DNA recombination and repair studies: Mechanistic assays of DNA repair processes.
Klein, Hannah L; Ang, Kenny K H; Arkin, Michelle R; Beckwitt, Emily C; Chang, Yi-Hsuan; Fan, Jun; Kwon, Youngho; Morten, Michael J; Mukherjee, Sucheta; Pambos, Oliver J; El Sayyed, Hafez; Thrall, Elizabeth S; Vieira-da-Rocha, João P; Wang, Quan; Wang, Shuang; Yeh, Hsin-Yi; Biteen, Julie S; Chi, Peter; Heyer, Wolf-Dietrich; Kapanidis, Achillefs N; Loparo, Joseph J; Strick, Terence R; Sung, Patrick; Van Houten, Bennett; Niu, Hengyao; Rothenberg, Eli.
Afiliación
  • Klein HL; New York University School of Medicine, Department of Biochemistry and Molecular Pharmacology, New York, NY 10016, USA.
  • Ang KKH; Small Molecule Discovery Center and Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94143, USA.
  • Arkin MR; Small Molecule Discovery Center and Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94143, USA.
  • Beckwitt EC; Program in Molecular Biophysics and Structural Biology, University of Pittsburgh, Pittsburgh, PA 15261, USA.
  • Chang YH; The University of Pittsburgh Cancer Institute, Hillman Cancer Center, Pittsburgh, PA 15213, USA.
  • Fan J; Institute of Biochemical Sciences, National Taiwan University, NO. 1, Section 4, Roosevelt Road, Taipei 10617, Taiwan.
  • Kwon Y; Biological Physics Research Group, Clarendon Laboratory, Department of Physics, University of Oxford, Oxford, OX1 3PU, UK.
  • Morten MJ; Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT 06520, USA.
  • Mukherjee S; Department of Biochemistry and Structural Biology, University of Texas Health San Antonio, San Antonio, Texas 78229, USA.
  • Pambos OJ; New York University School of Medicine, Department of Biochemistry and Molecular Pharmacology, New York, NY 10016, USA.
  • El Sayyed H; Department of Microbiology and Molecular Genetics, University of California, Davis, CA 95616, USA.
  • Thrall ES; Biological Physics Research Group, Clarendon Laboratory, Department of Physics, University of Oxford, Oxford, OX1 3PU, UK.
  • Vieira-da-Rocha JP; Biological Physics Research Group, Clarendon Laboratory, Department of Physics, University of Oxford, Oxford, OX1 3PU, UK.
  • Wang Q; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 250 Longwood Avenue, Boston, MA 02115, USA.
  • Wang S; Department of Microbiology and Molecular Genetics, University of California, Davis, CA 95616, USA.
  • Yeh HY; Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, IN 47405, USA.
  • Biteen JS; Ecole Normale Supérieure, Institut de Biologie de l'Ecole Normale Supérieure (IBENS), CNRS, INSERM, PSL Research University, 75005 Paris, France.
  • Chi P; Institut Jacques Monod, CNRS, UMR7592, University Paris Diderot, Sorbonne Paris Cité F-75205 Paris, France.
  • Heyer WD; Institute of Biochemical Sciences, National Taiwan University, NO. 1, Section 4, Roosevelt Road, Taipei 10617, Taiwan.
  • Kapanidis AN; Departments of Chemistry and Biophysics, University of Michigan, Ann Arbor, MI 48109, USA.
  • Loparo JJ; Institute of Biochemical Sciences, National Taiwan University, NO. 1, Section 4, Roosevelt Road, Taipei 10617, Taiwan.
  • Strick TR; Institute of Biological Chemistry, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei 11529, Taiwan.
  • Sung P; Department of Microbiology and Molecular Genetics, University of California, Davis, CA 95616, USA.
  • Van Houten B; Department of Molecular and Cellular Biology, University of California, Davis, CA 95616, USA.
  • Niu H; Biological Physics Research Group, Clarendon Laboratory, Department of Physics, University of Oxford, Oxford, OX1 3PU, UK.
  • Rothenberg E; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 250 Longwood Avenue, Boston, MA 02115, USA.
Microb Cell ; 6(1): 65-101, 2019 Jan 07.
Article en En | MEDLINE | ID: mdl-30652106
ABSTRACT
Genomes are constantly in flux, undergoing changes due to recombination, repair and mutagenesis. In vivo, many of such changes are studies using reporters for specific types of changes, or through cytological studies that detect changes at the single-cell level. Single molecule assays, which are reviewed here, can detect transient intermediates and dynamics of events. Biochemical assays allow detailed investigation of the DNA and protein activities of each step in a repair, recombination or mutagenesis event. Each type of assay is a powerful tool but each comes with its particular advantages and limitations. Here the most commonly used assays are reviewed, discussed, and presented as the guidelines for future studies.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Guideline Idioma: En Revista: Microb Cell Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Guideline Idioma: En Revista: Microb Cell Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos