Comparing polyvalent bacteriophage and bacteriophage cocktails for controlling antibiotic-resistant bacteria in soil-plant system.
Sci Total Environ
; 657: 918-925, 2019 Mar 20.
Article
en En
| MEDLINE
| ID: mdl-30677957
ABSTRACT
Antibiotic resistant pathogenic bacteria (ARPB) residual in soil-plant system has caused serious threat against public health and environmental safety. Being capable of targeted lysing host bacteria, phage therapy has been proposed as promising method to control the ARPB contamination in environments. In this study, microcosm trials were performed to investigate the impact of various phage treatments on the dissipation of tetracycline resistant Escherichia coli K-12 and chloramphenicol resistant Pseudomonas aeruginosa PAO1 in soil-carrot system. After 70â¯days of incubation, all the four phage treatments significantly decreased the abundance of the pathogenic bacteria and the corresponding antibiotic resistance genes (tetW and cmlA) in the soil-carrot system (pâ¯<â¯0.05), following the order of the cocktail phage treatment (phages ΦYSZ1â¯+â¯ΦYSZ2)â¯>â¯the polyvalent phage (ΦYSZ3 phage with broad host range) treatmentâ¯>â¯host-specific phage (ΦYSZ2 and ΦYSZ1) treatmentsâ¯>â¯the control. In addition, the polyvalent phage treatment also exerted positive impact on the diversity and stability of the bacterial community in the system, suggesting that this is an environmentally friendly technique with broad applications in the biocontrol of ARPB/ARGs in soil-plant system.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
3_ND
Problema de salud:
3_neglected_diseases
/
3_zoonosis
Asunto principal:
Pseudomonas aeruginosa
/
Microbiología del Suelo
/
Bacteriófagos
/
Farmacorresistencia Microbiana
/
Escherichia coli K12
Idioma:
En
Revista:
Sci Total Environ
Año:
2019
Tipo del documento:
Article
País de afiliación:
China