A novel label-free fluorescent detection of histidine based upon Cu2+-specific DNAzyme and hybridization chain reaction.
Spectrochim Acta A Mol Biomol Spectrosc
; 213: 42-47, 2019 Apr 15.
Article
en En
| MEDLINE
| ID: mdl-30682646
ABSTRACT
A novel label-free fluorescent sensor for histidine was developed based upon Cu2+-specific DNAzyme, hybridization chain reaction(HCR) and triplex DNA. Cu2+ can bind to the histidine, in the presence of histidine, leading to the inhibition of the cleavage of substrate strand of Cu2+-dependent DNAzyme, then the intact substrate strand trigger the HCR between H1 and H2. The HCR product can be recognized by triplex-forming oligonucleotide (TFO) through triplex formation and reported by the fluorescence of berberine, the fluorescence intensity of the sensing system was proportional to the concentration of histidine during the range of 5.7-455â¯nmolâ¯L-1, with a detection limit of 2.0â¯nmolâ¯L-1.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Cobre
/
ADN Catalítico
/
Histidina
/
Hibridación de Ácido Nucleico
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
Spectrochim Acta A Mol Biomol Spectrosc
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2019
Tipo del documento:
Article