Determination of the Activity of 1-Deoxy-D-Xylulose 5-Phosphate Synthase by Pre-column Derivatization-HPLC Using 1,2-Diamino-4,5-Methylenedioxybenzene as a Derivatizing Reagent.

Liang, Yan-Fei; Liu, Hui; Li, Heng; Gao, Wen-Yun

Liang, Yan-Fei; Liu, Hui; Li, Heng; Gao, Wen-Yun.

Afiliación

Liang YF; National Engineering Research Center for Miniaturized Detection Systems and College of Life Sciences, Northwest University, 229 North Taibai Road, Xi'an, 710069, Shaanxi, China.
Liu H; National Engineering Research Center for Miniaturized Detection Systems and College of Life Sciences, Northwest University, 229 North Taibai Road, Xi'an, 710069, Shaanxi, China.
Li H; National Engineering Research Center for Miniaturized Detection Systems and College of Life Sciences, Northwest University, 229 North Taibai Road, Xi'an, 710069, Shaanxi, China.
Gao WY; National Engineering Research Center for Miniaturized Detection Systems and College of Life Sciences, Northwest University, 229 North Taibai Road, Xi'an, 710069, Shaanxi, China. gaowenyun@nwu.edu.cn.

Protein J ; 38(2): 160-166, 2019 04.

Article en En | MEDLINE | ID: mdl-30707333

Asunto(s)

Proteínas Bacterianas/química; Transferasas/química; Cromatografía Líquida de Alta Presión/métodos; Escherichia coli/enzimología; Cinética; Pentosafosfatos/química; Fenilendiaminas/química

Palabras clave

1,2-Diamino-4,5-methylenedioxybenzene; 1-Deoxy-D-xylulose 5-phosphate synthase; Activity; HPLC; Non-mevalonate pathway

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Contexto en salud: 3_ND Problema de salud: 3_neglected_diseases / 3_zoonosis Asunto principal: Proteínas Bacterianas / Transferasas Idioma: En Revista: Protein J Asunto de la revista: BIOQUIMICA Año: 2019 Tipo del documento: Article País de afiliación: China

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