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Hydrogenated Diglucose Detergents for Membrane-Protein Extraction and Stabilization.
Guillet, Pierre; Mahler, Florian; Garnier, Kelly; Nyame Mendendy Boussambe, Gildas; Igonet, Sébastien; Vargas, Carolyn; Ebel, Christine; Soulié, Marine; Keller, Sandro; Jawhari, Anass; Durand, Grégory.
Afiliación
  • Guillet P; Equipe Chimie Bioorganique et Systèmes Amphiphiles , Institut des Biomolécules Max Mousseron (UMR 5247 UM-CNRS-ENSCM) & Avignon University , 301 rue Baruch de Spinoza , 84916 Avignon cedex 9, France.
  • Mahler F; CHEM2STAB , 301 rue Baruch de Spinoza , 84916 Avignon cedex 9, France.
  • Garnier K; Molecular Biophysics , Technische Universität Kaiserslautern (TUK) , Erwin-Schrödinger-Str. 13 , 67663 Kaiserslautern , Germany.
  • Nyame Mendendy Boussambe G; CHEM2STAB , 301 rue Baruch de Spinoza , 84916 Avignon cedex 9, France.
  • Igonet S; CALIXAR , 60 Avenue Rockefeller , 69008 Lyon , France.
  • Vargas C; Equipe Chimie Bioorganique et Systèmes Amphiphiles , Institut des Biomolécules Max Mousseron (UMR 5247 UM-CNRS-ENSCM) & Avignon University , 301 rue Baruch de Spinoza , 84916 Avignon cedex 9, France.
  • Ebel C; CHEM2STAB , 301 rue Baruch de Spinoza , 84916 Avignon cedex 9, France.
  • Soulié M; CHEM2STAB , 301 rue Baruch de Spinoza , 84916 Avignon cedex 9, France.
  • Keller S; CALIXAR , 60 Avenue Rockefeller , 69008 Lyon , France.
  • Jawhari A; Molecular Biophysics , Technische Universität Kaiserslautern (TUK) , Erwin-Schrödinger-Str. 13 , 67663 Kaiserslautern , Germany.
  • Durand G; Univ. Grenoble Alpes, CNRS, CEA, CNRS, IBS , F-38000 Grenoble , France.
Langmuir ; 35(12): 4287-4295, 2019 03 26.
Article en En | MEDLINE | ID: mdl-30767533
ABSTRACT
We report herein the design and synthesis of a novel series of alkyl glycoside detergents consisting of a nonionic polar headgroup that comprises two glucose moieties in a branched arrangement (DG), onto which octane-, decane-, and dodecanethiols were grafted leading to ODG, DDG, and DDDG detergents, respectively. Micellization in aqueous solution was studied by isothermal titration calorimetry, 1H NMR spectroscopy, and surface tensiometry. Critical micellar concentration values were found to decrease by a factor of ∼10 for each pair of methylene groups added to the alkyl chain, ranging from ∼0.05 to 9 mM for DDDG and ODG, respectively. Dynamic light scattering and analytical ultracentrifugation sedimentation velocity experiments were used to investigate the size and composition of the micellar aggregates, showing that the aggregation number significantly increased from ∼40 for ODG to ∼80 for DDDG. All new compounds were able to solubilize membrane proteins (MPs) from bacterial membranes, insect cells, as well as the Madin-Darby canine kidney cells. In particular, native human adenosine receptor (A2AR) and bacterial transporter (BmrA) were solubilized efficiently. Striking thermostability improvements of +13 and +8 °C were observed when ODG and DDG were, respectively, applied to wild-type and full-length A2AR. Taken together, this novel detergent series shows promising detergent potency for solubilization and stabilization of membrane proteins (MPs) and thus makes a valuable addition to the chemical toolbox available for extracting and handling these important but challenging MP targets.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Detergentes / Glucosa / Proteínas de la Membrana Idioma: En Revista: Langmuir Asunto de la revista: QUIMICA Año: 2019 Tipo del documento: Article País de afiliación: Francia

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Detergentes / Glucosa / Proteínas de la Membrana Idioma: En Revista: Langmuir Asunto de la revista: QUIMICA Año: 2019 Tipo del documento: Article País de afiliación: Francia
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