Structural dynamics of bacteriophage P22 infection initiation revealed by cryo-electron tomography.
Nat Microbiol
; 4(6): 1049-1056, 2019 06.
Article
en En
| MEDLINE
| ID: mdl-30886360
ABSTRACT
For successful infection, bacteriophages must overcome multiple barriers to transport their genome and proteins across the bacterial cell envelope. We use cryo-electron tomography to study the infection initiation of phage P22 in Salmonella enterica serovar Typhimurium, revealing how a channel forms to allow genome translocation into the cytoplasm. Our results show free phages that initially attach obliquely to the cell through interactions between the O antigen and two of the six tailspikes; the tail needle also abuts the cell surface. The virion then orients perpendicularly and the needle penetrates the outer membrane. The needle is released and the internal head protein gp7* is ejected and assembles into an extracellular channel that extends from the gp10 baseplate to the cell surface. A second protein, gp20, is ejected and assembles into a structure that extends the extracellular channel across the outer membrane into the periplasm. Insertion of the third ejected protein, gp16, into the cytoplasmic membrane probably completes the overall trans-envelope channel into the cytoplasm. Construction of a trans-envelope channel is an essential step during infection of Gram-negative bacteria by all short-tailed phages, because such virions cannot directly deliver their genome into the cell cytoplasm.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
3_ND
Problema de salud:
3_neglected_diseases
/
3_zoonosis
Asunto principal:
Salmonella typhimurium
/
Membrana Celular
/
Bacteriófago P22
/
Acoplamiento Viral
/
Internalización del Virus
/
Tomografía con Microscopio Electrónico
Idioma:
En
Revista:
Nat Microbiol
Año:
2019
Tipo del documento:
Article
País de afiliación:
Estados Unidos