Root canal dressings for revascularization influence in vitro mineralization of apical papilla cells.
J Appl Oral Sci
; 27: e20180396, 2019.
Article
en En
| MEDLINE
| ID: mdl-30994774
ABSTRACT
Endodontic revascularization is based on cell recruitment into the necrotic root canal of immature teeth after chemical disinfection. The clinical outcome depends on the ability of surviving cells from the apical tissue to differentiate and promote hard tissue deposition inside the dentinal walls. OBJECTIVE:
To investigate the effect of calcium hydroxide (CH) and modified triple antibiotic paste (mTAP - ciprofloxacin, metronidazole and cefaclor) on the viability and mineralization potential of apical papilla cells (APC) in vitro . MATERIAL ANDMETHODS:
APC cultures were kept in contact with CH or mTAP (250-1000 µg/mL) for 5 days, after which cell viability was assessed using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Next, APCs were subjected to CH or mTAP at 250 µg/mL for 5 days before inducing the differentiation assay. After 14 and 21 days, calcium deposition was assessed by the Alizarin Red S staining method, followed by elution and quantification using spectrophotometry. Data were analyzed using ANOVA followed by Tukey post hoc test.RESULTS:
CH induced cell proliferation, whereas mTAP showed significant cytotoxicity at all concentrations tested. APC treated with CH demonstrated improved mineralization capacity at 14 days, while, for mTAP, significant reduction on the mineralization rate was observed for both experimental periods (14 and 21 days).CONCLUSION:
Our findings showed that CH induces cell proliferation and improves early mineralization, whereas mTAP was found cytotoxic and reduced the mineralization potential in vitro of APCs.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Irrigantes del Conducto Radicular
/
Hidróxido de Calcio
/
Papila Dental
/
Antibacterianos
Tipo de estudio:
Evaluation_studies
Límite:
Humans
Idioma:
En
Revista:
J Appl Oral Sci
Asunto de la revista:
ODONTOLOGIA
Año:
2019
Tipo del documento:
Article
País de afiliación:
Brasil