Development of blastocyst complementation technology without contributions to gametes and the brain.
Exp Anim
; 68(3): 361-370, 2019 Aug 14.
Article
en En
| MEDLINE
| ID: mdl-30996149
ABSTRACT
In Japan, it is possible to generate chimeric animals from specified embryos by combining animal blastocysts with human pluripotent stem (PS) cells (animal-human PS chimera). However, the production of animal-human PS chimeras has been restricted because of ethical concerns, such as the development of human-like intelligence and formation of humanized gametes in the animals, owing to the contributions of human PS cells to the brain and reproductive organs. To solve these problems, we established a novel blastocyst complementation technology that does not contribute to the gametes or the brain. First, we established GFP-expressing mouse embryonic stem cells (G-mESCs) in which the Prdm14 and Otx2 genes were knocked out and generated chimeric mice by injecting them into PDX-1-deficient blastocysts. The results showed that the G-mESCs did not contribute to the formation of gametes and the brain. Therefore, in the PDX-1-deficient mice complemented by G-mESCs without the Prdm14 and Otx2 genes, the germline was not transmitted to the next generations. This approach could address concerns regarding the development of both human gametes and a human-like brain upon mouse blastocyst complementation using human stem cells.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Blastocisto
/
Diferenciación Celular
/
Técnicas de Cultivo de Embriones
/
Transferencia de Embrión
/
Células Madre Embrionarias de Ratones
Aspecto:
Ethics
Límite:
Animals
País/Región como asunto:
Asia
Idioma:
En
Revista:
Exp Anim
Asunto de la revista:
MEDICINA VETERINARIA
Año:
2019
Tipo del documento:
Article
País de afiliación:
Japón