Invited review: Quantifying proton exchange from chemical reactions - Implications for the biochemistry of metabolic acidosis.
Comp Biochem Physiol A Mol Integr Physiol
; 235: 29-45, 2019 09.
Article
en En
| MEDLINE
| ID: mdl-31071454
ABSTRACT
Given that the chemistry of lactate production disproves the existence of a lactic acidosis, there is a need to further reveal and explain the importance of the organic and computational chemistry of pH dependent competitive cation fractional (~) proton (H+) exchange (~H+e). An additional importance of this knowledge is that it could potentially contradict the assumption of the Stewart approach to the physico-chemical theory of acid-base balance. For example, Stewart proposed that chemical reaction and pH dependent H+ dissociation and association do not directly influence the pH of cellular and systemic body fluids. Yet at the time of Stewart's work, there were no data that quantified the H+ exchange during chemical reactions, or from pH dependent metabolite H+ association or dissociation. Consequently, the purpose of this review and commentary was three-fold; 1) to provide explanation of pH dependent competitive cation ~H+e exchange; 2) develop a model of and calculate new data of substrate flux in skeletal muscle during intense exercise; and 3) then combine substrate flux data with the now known ~H+e from chemical reactions of non-mitochondrial energy catabolism to quantify chemical reaction and metabolic pathway ~H+e. The results of purpose 3 were that ~H+ release for the totality of cytosolic energy catabolismâ¯=â¯-187.2â¯mmol·L-1, where total glycolytic ~H+teâ¯=â¯-85.0â¯mmol·L-1. ATP hydrolysis had a ~H+teâ¯=â¯-43.1â¯mmol·L-1. Lactate production provided the largest metabolic ~H+ buffering with a ~H+teâ¯=â¯44.5â¯mmol·L-1. The total ~H+ release to La ratioâ¯=â¯4.25. The review content and research results of this manuscript should direct science towards new approaches to understanding the cause and source of H+e during metabolic acidosis and alkalosis.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Protones
/
Acidosis
/
Líquidos Corporales
/
Alcalosis
Límite:
Humans
Idioma:
En
Revista:
Comp Biochem Physiol A Mol Integr Physiol
Asunto de la revista:
BIOLOGIA MOLECULAR
/
FISIOLOGIA
Año:
2019
Tipo del documento:
Article