Transferrin isoforms in cerebrospinal fluid and their relation to neurological diseases.
Proc Jpn Acad Ser B Phys Biol Sci
; 95(5): 198-210, 2019.
Article
en En
| MEDLINE
| ID: mdl-31080188
ABSTRACT
Iron plays many important roles in the brain, including involvement in myelination, neurotransmission and electron transfer in the respiratory chain. Transferrin (Tf), an iron transporter, is mainly biosynthesized in the liver, but can also be biosynthesized in the brain; i.e., by oligodendrocytes and the choroid plexus, a cerebrospinal fluid (CSF) producing tissue. The CSF contains two Tf isoforms, brain-type Tf and serum-type Tf, which differ in their glycan structures. Brain-type Tf is uniquely glycolsylated with biantennary asialo- and agalacto-complex type N-glycans that carry bisecting ß1,4-GlcNAc and core α1,6-Fuc. The glycans of serum-type Tf in the CSF are similar to those of Tf in serum. Biochemical analyses reveal that the apparent molecular size of brain-type Tf is smaller than that of serum-type Tf, and that hydrophobic patches are exposed on brain-type Tf as demonstrated by hydrophobic probe binding studies. We found that brain-type Tf levels were decreased in idiopathic normal pressure hydrocephalus, in which CSF production is suspected to decrease, while brain-type Tf increased in spontaneous intracranial hypotension, in which CSF production is suspected to increase. These results suggest that brain-type Tf could be a biomarker of altered CSF production.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Transferrina
/
Enfermedades del Sistema Nervioso
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Proc Jpn Acad Ser B Phys Biol Sci
Asunto de la revista:
BIOLOGIA
/
FISIOLOGIA
Año:
2019
Tipo del documento:
Article