EGFR is required for Wnt9a-Fzd9b signalling specificity in haematopoietic stem cells.
Nat Cell Biol
; 21(6): 721-730, 2019 06.
Article
en En
| MEDLINE
| ID: mdl-31110287
ABSTRACT
Wnt signalling drives many processes in development, homeostasis and disease; however, the role and mechanism of individual ligand-receptor (Wnt-Frizzled (Fzd)) interactions in specific biological processes remain poorly understood. Wnt9a is specifically required for the amplification of blood progenitor cells during development. Using genetic studies in zebrafish and human embryonic stem cells, paired with in vitro cell biology and biochemistry, we determined that Wnt9a signals specifically through Fzd9b to elicit ß-catenin-dependent Wnt signalling that regulates haematopoietic stem and progenitor cell emergence. We demonstrate that the epidermal growth factor receptor (EGFR) is required as a cofactor for Wnt9a-Fzd9b signalling. EGFR-mediated phosphorylation of one tyrosine residue on the Fzd9b intracellular tail in response to Wnt9a promotes internalization of the Wnt9a-Fzd9b-LRP signalosome and subsequent signal transduction. These findings provide mechanistic insights for specific Wnt-Fzd signals, which will be crucial for specific therapeutic targeting and regenerative medicine.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Pez Cebra
/
Células Madre Hematopoyéticas
/
Receptores de Neurotransmisores
/
Proteínas de Pez Cebra
/
Proteínas Wnt
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Nat Cell Biol
Año:
2019
Tipo del documento:
Article
País de afiliación:
Estados Unidos