ZNF492 and GPR149 methylation patterns as prognostic markers for clear cell renal cell carcinoma: Arraybased DNA methylation profiling.
Oncol Rep
; 42(1): 453-460, 2019 Jul.
Article
en En
| MEDLINE
| ID: mdl-31115548
ABSTRACT
The present study aimed to identify novel methylation markers of clear cell renal cell carcinoma (ccRCC) using microarray methylation analysis and evaluate their prognostic relevance in patient samples. To identify cancerspecific methylated biomarkers, microarray profiling of ccRCC samples from our institute (n=12) and The Cancer Genome Atlas (TCGA) database (n=160) were utilized, and the prognostic relevance of candidate genes were investigated in another TCGA dataset (n=153). For validation, pyrosequencing analyses with ccRCC samples from our institute (n=164) and another (n=117) were performed and the potential clinical application of selected biomarkers was examined. We identified 22 CpG island loci that were commonly hypermethylated in ccRCC. KaplanMeier analysis of TCGA data indicated that only 4/22 loci were significantly associated with disease progression. In the internal validation set, KaplanMeier analysis revealed that hypermethylation of two loci, zinc finger protein 492 (ZNF492) and G proteincoupled receptor 149 (GPR149), was significantly associated with shorter timetoprogression. Multivariate Cox regression models revealed that hypermethylation of ZNF492 [hazard ratio (HR), 5.44; P=0.001] and GPR149 (HR, 7.07; P<0.001) may be independent predictors of tumor progression. Similarly, the methylation status of these two genes was significantly associated with poor outcomes in the independent external validation cohort. Collectively, the present study proposed that the novel methylation markers ZNF492 and GPR149 could be independent prognostic indicators in patients with ccRCC.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Factores de Transcripción
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Carcinoma de Células Renales
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Biomarcadores de Tumor
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Análisis de Secuencia de ADN
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Receptores Acoplados a Proteínas G
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Proteínas de Unión al ADN
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Neoplasias Renales
Tipo de estudio:
Prognostic_studies
Límite:
Adult
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Aged
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Aged80
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
Oncol Rep
Asunto de la revista:
NEOPLASIAS
Año:
2019
Tipo del documento:
Article