Molecular characterization of Hymenolepis nana based on nuclear rDNA ITS2 gene marker.
Afr Health Sci
; 19(1): 1346-1352, 2019 Mar.
Article
en En
| MEDLINE
| ID: mdl-31148960
ABSTRACT
INTRODUCTION:
Hymenolepis nana is a zoonotic tapeworm with widespread distribution. The goal of the present study was to identify the parasite in the specimens collected from NorthWestern regions of Iran using PCR-sequencing method.METHODS:
A total of 1521 stool samples were collected from the study individuals. Initially, the identification of hymenolepis nana was confirmed by parasitological method including direct wet-mount and formalin-ethyl acetate concentration methods. Afterward, PCR-sequencing analysis of ribosomal ITS2 fragment was targeted to investigate the molecular identification of the parasite.RESULTS:
Overall, 0.65% (10/1521) of the isolates were contaminated with H. nana in formalin-ethyl acetate concentration. All ten isolates were succefully amplified by PCR and further sequenced. The determined sequences were deposited in GenBank under the accession numbers MH337810 -MH337819.CONCLUSION:
Our results clarified the presence of H. nana among the patients in the study areas. In addition, the molecular technique could be accessible when the human eggs are the only sources available to identify and diagnose the parasite.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
3_ND
Problema de salud:
3_helminthiasis
/
3_neglected_diseases
/
3_zoonosis
Asunto principal:
ADN Ribosómico
/
Hymenolepis nana
/
Himenolepiasis
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Animals
/
Humans
País/Región como asunto:
Asia
Idioma:
En
Revista:
Afr Health Sci
Asunto de la revista:
MEDICINA
/
SERVICOS DE SAUDE
Año:
2019
Tipo del documento:
Article
País de afiliación:
Irán