Self-powered and self-signalled autonomous electrochemical biosensor applied to cancinoembryonic antigen determination.
Biosens Bioelectron
; 140: 111320, 2019 Sep 01.
Article
en En
| MEDLINE
| ID: mdl-31150987
ABSTRACT
This work describes a novel and disruptive electrochemical biosensing device that is self-powered by light and self-signalled by an optical readout. Electrical energy requirements are ensured by a photovoltaic cell that is a dye sensitized solar cell (DSSC), in which one of the electrodes is the biosensing unit. The readout converts electrical energy into colour by an electrochromic cell and signals the concentration dependent event. This device was designed to target a cancer biomarker, cancinoembryonic antigen (CEA). In brief, the sensing unit was assembled on a conductive glass substrate with a highly conductive poly(3,4-ethylenedioxythiophene) (PEDOT) layer, using a molecularly-imprinted polymer of polypyrrol (PPy) as biorecognition element. This sensing unit acted as the counter electrode (CE) of the DSSC, generating a hybrid device with a maximum power conversion efficiency of 3.45% for a photoanode area of 0.7â¯cm2. The hybrid DSSC/biosensor had an electrical output that was CEA concentration dependent from 100â¯ng/mL to 100 µg/mL, with a limit detection of 0.14â¯ng/mL in human urine samples. The electrochromic cell consisted of a PEDOT-based material and showed a colour gradient change for CEA concentrations, ranging from 0.1â¯ng/mL to 100 µg/mL. Overall, this self-powered and self-signalled set-up is equipment free and particularly suitable for point-of-care analysis (POC), being able to screen CEA in real samples and differentiating critical concentrations for establishing a diagnosis. It holds the potential to provide clinical relevant data anywhere, in a fully independent manner.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Técnicas Biosensibles
/
Antígeno Carcinoembrionario
Tipo de estudio:
Evaluation_studies
Límite:
Humans
Idioma:
En
Revista:
Biosens Bioelectron
Asunto de la revista:
BIOTECNOLOGIA
Año:
2019
Tipo del documento:
Article
País de afiliación:
Portugal