The application of Compont gel in chronic obstructive jaundice rats model.
Acta Cir Bras
; 34(5): e201900504, 2019.
Article
en En
| MEDLINE
| ID: mdl-31166460
ABSTRACT
PURPOSE:
To establish a new rat model, the pathogenesis of which is closer to the clinical occurrence of chronic obstructive jaundice with liver fibrosis.METHODS:
90 SD rats were randomly divided into 3 groups. Group A common bile duct ligation, group B common bile duct injection compont and group C injection saline. The serum of three groups was extracted, and the liver function was detected by ELISA. HE staining, Masson staining and immunohistochemistry were used to detect liver pathology.RESULTS:
Group B showed a fluctuant development of jaundice, obstructive degree reached a peak at 2 weeks, and decreased from 3 weeks. HA, LA and PCIII were significantly higher than control group. 3 weeks after surgery, liver tissue fibrosis occurred in group B, and a wide range of fiber spacing was formed at 5 weeks. Immunohistochemistry showed that hepatic stellate cells were more active than the control group.CONCLUSION:
Intra-biliary injection of Compont gel is different from the classic obstructive jaundice animal model caused by classic bile duct ligation, which can provide an ideal rat model of chronic obstructive jaundice with liver fibrosis.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
6_ODS3_enfermedades_notrasmisibles
Problema de salud:
6_cirrhosis
/
6_digestive_diseases
Asunto principal:
Conductos Biliares
/
Ictericia Obstructiva
/
Modelos Animales de Enfermedad
/
Geles
/
Cirrosis Hepática
Tipo de estudio:
Evaluation_studies
/
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Acta Cir Bras
Año:
2019
Tipo del documento:
Article
País de afiliación:
China