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Heterobasidion-growth inhibiting Bacillus subtilis A18 exhibits medium- and age-dependent production of lipopeptides.
Azeem, Muhammad; Barba-Aliaga, Marina; Borg-Karlson, Anna Karin; Terenius, Olle; Broberg, Anders; Rajarao, Gunaratna Kuttuva.
Afiliación
  • Azeem M; Department of Chemistry, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, SE-100 44 Stockholm, Sweden; Department of Chemistry, COMSATS University Islamabad, Abbottabad Campus, Abbottabad 22060, Pakistan. Electronic address: muhazeem@cuiatd.ed
  • Barba-Aliaga M; Department of Industrial Biotechnology, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, SE-100 44 Stockholm, Sweden.
  • Borg-Karlson AK; Department of Chemistry, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, SE-100 44 Stockholm, Sweden; Division of Organic Chemistry, Institute of Technology, Tartu University, Tartu 50411, Estonia.
  • Terenius O; Department of Ecology, Swedish University of Agricultural Sciences (SLU), Box 7044, SE-750 07 Uppsala, Sweden; Department of Cell and Molecular Biology, Uppsala University, BMC, Box 596, SE-751 24, Uppsala, Sweden.
  • Broberg A; Department of Molecular Sciences, Uppsala BioCenter, Swedish University of Agricultural Sciences (SLU), Box 7015, SE-750 07 Uppsala, Sweden.
  • Rajarao GK; Department of Industrial Biotechnology, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, SE-100 44 Stockholm, Sweden. Electronic address: grk@kth.se.
Microbiol Res ; 223-225: 129-136, 2019.
Article en En | MEDLINE | ID: mdl-31178045
Heterobasidion annosum s.s. and H. parviporum are severe pathogens of conifers causing butt rot and root rot thus reducing the economic value of timber. Here, the antifungal activity of Bacillus subtilis isolate A18 against these two Heterobasidion species was investigated. Five different culture media with different culture age were investigated to study the effect of substrate composition and culture age for metabolite production. Bacterial cultures and cell-free culture filtrates were tested for antifungal activity. Inhibition of fungal growth was analysed using the agar disc-diffusion method. MALDI-TOF and LC-HRMS analyses were used to identify the antifungal metabolites. Substrate composition and age of culture were found to be active variables with direct effect on the antifungal activity of bacterial culture extracts. High anti-fungal activity was observed when B. subtilis was cultured in PDB, SGB and LB media for four days. Mass-spectrometry analysis showed the presence of lipopeptides in culture filtrates identified as members of the surfactins, polymixins, kurstakins and fengycins. A culture filtrate containing fengycin-type lipopeptides showed the highest bioactivity against Heterobasidion species. Bacterial cultures had higher bioactivity compared to their respective cell free culture filtrates. The results of the present study suggest that B. subtilis A18 is a powerful biocontrol agent against Heterobasidion infections of tree wounds and stumps.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacillus subtilis / Basidiomycota / Lipopéptidos / Agentes de Control Biológico / Antifúngicos Tipo de estudio: Prognostic_studies Idioma: En Revista: Microbiol Res Asunto de la revista: MICROBIOLOGIA / SAUDE AMBIENTAL Año: 2019 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacillus subtilis / Basidiomycota / Lipopéptidos / Agentes de Control Biológico / Antifúngicos Tipo de estudio: Prognostic_studies Idioma: En Revista: Microbiol Res Asunto de la revista: MICROBIOLOGIA / SAUDE AMBIENTAL Año: 2019 Tipo del documento: Article
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