LC-MS/MS method for quantification of 3,4-dihydroxyphenylglycol, a norepinephrine metabolite in plasma and brain regions.
Bioanalysis
; 11(10): 971-986, 2019 May.
Article
en En
| MEDLINE
| ID: mdl-31218903
ABSTRACT
Aim:
To evaluate suitability of the LC-MS/MS method to quantify 3,4-dihydroxyphenylglycol (DHPG) that is used as a biomarker for monoamine oxidase (MAO) inhibition.Methods:
DHPG was extracted using alumina basic cartridges and quantified on a triple quadrupole mass spectrometer using negative electrospray ionization, without the use of derivatization reagents.Results:
Modulation of DHPG levels was observed following administration of selective and nonselective MAO inhibitors and results were in correlation with historical MAO inhibition potential of compounds.Conclusion:
The proposed method is sensitive enough to measure plasma DHPG levels and DHPG can be used as a biomarker to assess MAO inhibition potential of new therapeutic agents.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Análisis Químico de la Sangre
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Encéfalo
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Norepinefrina
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Cromatografía Liquida
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Espectrometría de Masas en Tándem
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Metoxihidroxifenilglicol
Límite:
Animals
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Humans
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Male
Idioma:
En
Revista:
Bioanalysis
Año:
2019
Tipo del documento:
Article
País de afiliación:
India