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Carotenoids from UV-resistant Antarctic Microbacterium sp. LEMMJ01.
Reis-Mansur, Maria Cristina P P; Cardoso-Rurr, Janine S; Silva, Josemar V Maiworm Abreu; de Souza, Gabriela Rodrigues; Cardoso, Verônica da Silva; Mansoldo, Felipe Raposo Passos; Pinheiro, Yuri; Schultz, Júnia; Lopez Balottin, Luciene B; da Silva, Antonio Jorge Ribeiro; Lage, Claudia; Dos Santos, Elisabete Pereira; Rosado, Alexandre Soares; Vermelho, Alane Beatriz.
Afiliación
  • Reis-Mansur MCPP; BIOINOVAR - Biocatalysis, Bioproducts and Bioenergy, Paulo de Góes Institute of Microbiology, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil.
  • Cardoso-Rurr JS; LaRBio - Radiations and Biology Laboratory, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil.
  • Silva JVMA; Labio/Dimav/Inmetro - Laboratory of Tissue Bioengineering/Directorate of Metrology Applied to Life Sciences/National Institute of Metrology, Quality and Technology, Duque de Caxias, Brazil.
  • de Souza GR; Research Institute for Natural Products, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil.
  • Cardoso VDS; BIOINOVAR - Biocatalysis, Bioproducts and Bioenergy, Paulo de Góes Institute of Microbiology, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil.
  • Mansoldo FRP; BIOINOVAR - Biocatalysis, Bioproducts and Bioenergy, Paulo de Góes Institute of Microbiology, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil.
  • Pinheiro Y; LEMM - Laboratory of Microbial Molecular Ecology, Paulo de Góes Institute of Microbiology, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil.
  • Schultz J; LEMM - Laboratory of Microbial Molecular Ecology, Paulo de Góes Institute of Microbiology, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil.
  • Lopez Balottin LB; Labio/Dimav/Inmetro - Laboratory of Tissue Bioengineering/Directorate of Metrology Applied to Life Sciences/National Institute of Metrology, Quality and Technology, Duque de Caxias, Brazil.
  • da Silva AJR; Research Institute for Natural Products, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil.
  • Lage C; LaRBio - Radiations and Biology Laboratory, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil.
  • Dos Santos EP; Faculty of Pharmacy, Galenico Development Laboratory, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil.
  • Rosado AS; LEMM - Laboratory of Microbial Molecular Ecology, Paulo de Góes Institute of Microbiology, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil.
  • Vermelho AB; BIOINOVAR - Biocatalysis, Bioproducts and Bioenergy, Paulo de Góes Institute of Microbiology, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil. abvermelho@micro.ufrj.br.
Sci Rep ; 9(1): 9554, 2019 07 02.
Article en En | MEDLINE | ID: mdl-31266976
ABSTRACT
The Microbacterium sp. LEMMJ01 isolated from Antarctic soil does not belong to any of the nearest species identified in the RDP database. Under UV radiation (A, B and C wavebands) the survival fractions of Microbacterium sp. cells were much higher compared with wild-type E. coli K12A15. Especially remarkable for an Antarctic bacterium, an expressive resistance against high UV-B doses was observed. The increased survival of DNA repair-proficient E. coli grown overnight added of 0.1 mg/ml or 1 mg/ml of the whole pigment extract produced by Microbacterium sp. revealed that part of the resistance of Microbacterium sp. against UV-B radiation seems to be connected with photoprotection by its pigments. Scanning electron microscopy revealed that UV-A and UV-B ensued membrane alterations only in E. coli. The APCI-MS fingerprints revealed the diagnostic ions for neurosporene (m/z 580, 566, 522, 538, and 524) synergism for the first time in this bacterium by HPLC-MS/MS analysis. Carotenoids also were devoid of phototoxicity and cytotoxicity effects in mouse cells and in human keratinocytes and fibroblasts.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Contexto en salud: 3_ND Problema de salud: 3_neglected_diseases / 3_zoonosis Asunto principal: Tolerancia a Radiación / Rayos Ultravioleta / Carotenoides / Actinobacteria Límite: Humans Idioma: En Revista: Sci Rep Año: 2019 Tipo del documento: Article País de afiliación: Brasil
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Contexto en salud: 3_ND Problema de salud: 3_neglected_diseases / 3_zoonosis Asunto principal: Tolerancia a Radiación / Rayos Ultravioleta / Carotenoides / Actinobacteria Límite: Humans Idioma: En Revista: Sci Rep Año: 2019 Tipo del documento: Article País de afiliación: Brasil