Effect of endoplasmic reticulum calcium on paraquatinduced apoptosis of human lung type II alveolar epithelial A549 cells.
Mol Med Rep
; 20(3): 2419-2425, 2019 Sep.
Article
en En
| MEDLINE
| ID: mdl-31322172
ABSTRACT
The present study aimed to explore the role of endoplasmic reticulum calcium (ER Ca2+) in the apoptosis of human lung type II alveolar epithelial A549 cells induced by paraquat (PQ) in vitro. PQ significantly elevated the intracellular Ca2+ concentration. Treatment with the Ca2+ATPase inhibitor thapsigargin significantly increased PQinduced cytotoxicity, elevated the intracellular level of Ca2+, and increased the apoptosis rate, the protein expression of glucoseregulated protein 78 (GRP78) and C/EBP homologous protein (CHOP), and the activities of caspase7 and caspase12 in PQtreated cells. By contrast, treatment with heparin, an inositol 1,4,5triphosphate receptor inhibitor, remarkably attenuated cytotoxicity and decreased the intracellular level of Ca2+, the apoptosis rate and the expression levels of GRP78, CHOP and Caspases. In conclusion, PQ impaired the regulating function of ER Ca2+ and resulted in an excessive increase of intracellular Ca2+. Therefore, influencing the Ca2+ signaling in the ER influenced the apoptosis of A549 cells via the ER stress pathway.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Paraquat
/
Calcio
/
Apoptosis
/
Retículo Endoplásmico
/
Células Epiteliales Alveolares
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Herbicidas
Límite:
Humans
Idioma:
En
Revista:
Mol Med Rep
Año:
2019
Tipo del documento:
Article