Enhanced reaction with Vicia graminea lectin and exposed terminal N-acetyl-D-glucosaminyl residues on a sample of human red cells with Hb M-Hyde Park.
Transfusion
; 28(6): 549-55, 1988.
Article
en En
| MEDLINE
| ID: mdl-3143169
ABSTRACT
A sample of polyagglutinable red cells was obtained from a healthy individual (group O, N) possessing a hemoglobin (Hb) variant called Hb M-Hyde Park. The sialic acid content of the individual's red cells is 90 percent of normal, and his cells are agglutinated by monoclonal but not lectin anti-Tn, a panel of lectins specific for N-acetylgalactosamine (or galactose), and N-acetylglucosamine. Enhanced agglutination reactions were obtained with Vicia graminea, Ulex europaeus, and human anti-I and -i. Using various enzyme treatments and different methods of labeling cell surface components, two defective cell membrane sites have been identified one associated with the O-linked oligosaccharides on sialoglycoproteins and the other associated with exposed N-acetylglucosaminyl residues located on membrane components of apparent molecular weights 88,000 to 130,000 and 46,000 to 73,000 (probably the Band 3 and Band 4.5 regions, respectively).
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Acetilglucosamina
/
Hemoglobina M
/
Hemoglobinas Anormales
/
Lectinas de Plantas
/
Glucosamina
/
Glicósido Hidrolasas
/
Lectinas
/
Proteínas de la Membrana
Límite:
Humans
Idioma:
En
Revista:
Transfusion
Año:
1988
Tipo del documento:
Article
País de afiliación:
Reino Unido