ATMP-induced three-dimensional conductive polymer hydrogel scaffold for a novel enhanced solid-state electrochemiluminescence biosensor.
Biosens Bioelectron
; 143: 111601, 2019 Oct 15.
Article
en En
| MEDLINE
| ID: mdl-31442752
ABSTRACT
Reliable and sensitive detection of xanthine has important medical and biological significance. In this work, a novel three-dimensional (3D) conductive polymer hydrogel of polyaniline (PAni) was feasibly prepared using aniline (Ani), amino trimethylene phosphonic acid (ATMP) and ammonium persulfate ((NH4)2S2O8) as monomer, gelatinizing agent and oxidizing agent, respectively. Protonation of aniline can be achieved by ATMP, inducing good conductivity of the obtained hydrogel. ATMP remained the chelating abilities in the conductive hydrogel, enabling further immobilization with silver nanoparticles (AgNPs) functionalized by a luminol derivative, N-(aminobutyl)-N-(ethylisoluminol) (ABEI). ABEI-Ag@PAni-ATMP exhibited an enhanced performance of solid-state electrochemiluminescence (ECL). Integrated with xanthine oxidase (XOD), the proposed biosensor can be applied in the detection of xanthine via in-situ generated hydrogen peroxide (H2O2), and present a low detection limit of 9.6â¯nM, a wide linear range (from 0.01 to 200⯵M) and excellent stability.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Técnicas Biosensibles
/
Xantina
/
Técnicas Electroquímicas
Idioma:
En
Revista:
Biosens Bioelectron
Asunto de la revista:
BIOTECNOLOGIA
Año:
2019
Tipo del documento:
Article
País de afiliación:
China