ABILITY TO FORM BIOFILMS BY PYELONEPHRITIS CAUSATIVE AGENTS IN CHILDREN.

ABSTRACT

The work is dedicated to the study of biofilms formation process by main pyelonephritis causative agents in children in vitro using methods of light, fluorescent and scanning microscopy. To study biofilms formation bacteria were cultivated in liquid substratum on glass in polystyrene Petri dishes d=40mm. The study demonstrated that all isolates formed biofilms. Adhesion of bacteria planktonic forms took place on the first stage, intracellular matrix formation took place on the second stage, and biofilms formation took place on the third stage. During the study of E. coli and Proteus sрр bacteria preparations with the use of scanning and light microscopy ordered bacteria arrangement was seen in the form of separate structures or tiny clusters of bacterial cells united by matrix. During the study of the ability to form P. aeruginosa isolates biofilms with the help of scanning microscopy it was stated that the adhesion of separate bacterial cells occurs by conglomerates formation surrounded by matrix with further biofilms formation. Bacterial cells in the form of dense elongated sticks were seen under the film. P. аeruginosa isolates daily biofilms were stated to have dense structure in the form of gel. Packed biofilms areas with cells clusters with good fluorescence were found with the help of fluorescent microscopy. During daily K.рneumoniae isolates biofilms study by methods of scanning and fluorescent microscopy it was found that K.рneumoniae biofilms were covered with dense matrix and riddled with multiple canals in the form of apertures. During morphological peculiarities study of E. faecalis isolates biofilms formation with the use of scanning and fluorescent microscopy it was found that bacterial cells were densely packed and united by intracellular matrix under which bacteria of spherical shape were seen. Thus biofilms, the nature of which depends on the type of bacteria, are formed on the surface of conglomerates consisting of bacterial cells. Peculiarities of course and appearance of pyelonephritis chronic form and relapses in children is explained by biofilms formation.